Multiphoton microscopy (MPM) is a promising non‐invasive imaging tool for discriminating benign nevi from melanoma. In this study, we establish a MPM morphologic catalogue of common nevi, information that will be critical in devising strategies to distinguish them from nevi that are evolving to melanoma that may present with more subtle signs of malignancy. Thirty common melanocytic nevi were imaged in vivo using MPM. Quantitative parameters that can distinguish between different types of nevi were developed and confirmed by examining the histology of eleven of the imaged nevi. MPM features of nevi examined included cytologic morphology of melanocytes in the epidermis and dermis, the size and distribution of nevomelanocytes both within and around nests, the size of rete ridges, and the presence of immune cells in the dermis. Distinguishing features include cytological morphology, the size of nevomelanocytes, the size of nevomelanocyte nests, and the distribution of nevomelanocytes. Notably, these distinguishing characteristics were not easily appreciated in fixed tissues, highlighting essential differences in the morphology of live skin. Taken together, this work provides a morphologic compendium of normal nevi, information that will be critical in future studies directed at identifying melanocytic nevi that are evolving to melanoma.

中文翻译：

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通过多光子显微镜进行的非侵入性光学活检可识别常见黑素细胞痣的活体形态。

多光子显微镜（MPM）是一种很有前途的非侵入性成像工具，用于区分良性痣和黑色素瘤。在这项研究中，我们建立了常见痣的 MPM 形态学目录，这些信息对于制定策略以将它们与正在演变为可能呈现更微妙的恶性肿瘤迹象的黑色素瘤的痣区分开来至关重要。使用 MPM 在体内对 30 个常见的黑素细胞痣进行成像。通过检查 11 个成像痣的组织学，开发和确认了可以区分不同类型痣的定量参数。检查的痣的 MPM 特征包括表皮和真皮中黑素细胞的细胞学形态、巢内和巢周围的黑素细胞的大小和分布、网脊的大小以及真皮中免疫细胞的存在。区别特征包括细胞形态学、黑素细胞的大小、黑素细胞巢的大小和黑素细胞的分布。值得注意的是，这些区别特征在固定组织中不容易被理解，突出了活体皮肤形态的本质差异。总之，这项工作提供了正常痣的形态学纲要，这些信息对于未来旨在识别正在演变为黑色素瘤的黑色素细胞痣的研究至关重要。