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Amperometric Biosensor for Brucella Testing through Molecular Orientation Technology in Combination with Signal Amplification Technology
ChemElectroChem ( IF 4 ) Pub Date : 2020-06-03 , DOI: 10.1002/celc.202000569
Hongshuo Chen 1, 2 , Chuanjin Cui 2 , Xuegang Ma 3 , Wei Yang 1 , Yueming Zuo 1
Affiliation  

Brucellosis is a global zoonotic disease causing abortion and infertility in animals. Based on an oriented immobilization technique of using a biomolecule in combination with a signal amplification technology, a novel biosensor for Brucella detection was designed. Graphene oxide/polypyrrole nanohybrids (GO/Ppy) modified a screen‐printed gold electrode through co‐electrodeposition for the oriented immobilization of a primary antibody (Ab1). Ab1 was successfully immobilized by using cyclic voltammetry (CV). A new second antibody nanoprobe GO/Fe3O4/MB/Ab2 (graphene oxide/nano‐iron oxide/methylene blue/Ab2) was accomplished by using physical and chemical methods to amplify the electrical signal. The successful fabrication of the biosensor was characterized by using SEM, AFM, Raman, and FTIR spectroscopy. The prepared biosensor was also tested in the Brucella solution by using differential pulse voltammetry (DPV). The results showed that the corresponding current was positively correlated with the logarithm of Brucella concentration, with a low detection limit of 2.2×102 CFU/mL. Moreover, the results of the biosensor‘s reproducibility, repeatability, specificity and standard recovery were satisfactory. New methodologies in fabricating the biosensor may be applied to detect other harmful microorganisms.

中文翻译:

通过分子定向技术与信号放大技术相结合的用于布鲁氏菌检测的安培生物传感器

布鲁氏菌病是一种导致动物流产和不育的全球人畜共患疾病。基于将生物分子与信号放大技术结合使用的定向固定技术,设计了一种用于布鲁氏菌检测的新型生物传感器。氧化石墨烯/聚吡咯纳米杂化物(GO / Ppy)通过共电沉积修饰了丝网印刷的金电极,用于一抗的定向固定(Ab 1)。通过使用循环伏安法(CV)成功固定了Ab 1。一种新的第二抗体纳米探针GO / Fe 3 O 4 / MB / Ab 2(氧化石墨烯/纳米氧化铁/亚甲基蓝/ Ab 2)是通过使用物理和化学方法放大电信号来完成的。通过使用SEM,AFM,拉曼和FTIR光谱对生物传感器的成功制造进行了表征。还通过使用差分脉冲伏安法(DPV)在Brucella溶液中测试了制备的生物传感器。结果表明,相应的电流与布鲁氏菌浓度的对数呈正相关,最低检出限为2.2×10 2  CFU / mL。此外,生物传感器的可重复性,可重复性,特异性和标准回收率的结果令人满意。制造生物传感器的新方法可以应用于检测其他有害微生物。
更新日期:2020-06-25
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