Phenolics, antioxidant activities, and antiproliferative properties of brown Hypsizygus marmoreus (brown HM) and white Hypsizygus marmoreus (white HM) were compared. The results showed that the contents of (+)-catechin, gallic acid, and protocatechuic acid of brown HM were higher than those of white HM. Moreover, brown HM had greater cellular antioxidant activity (CAA), peroxyl radical scavenging capacity (PSC), and oxygen radical absorbance capacity (ORAC) values than white HM, which demonstrated that brown HM presented a stronger antioxidant capacity. Both of brown HM and white HM showed remarkable antiproliferative activities against HepG2 cells and brown HM was proven to be the more effective. The flow cytometry results revealed that both of brown HM and white HM could induce G1 arrest and cell apoptotics in a dose-dependent manner. In addition, CyclinD1, CDK4, and Bcl-2 mRNA expression levels were downregulated with the treatment of brown HM or white HM. Taken together, our study revealed that brown HM afforded better antioxidant and antiproliferative activities than white HM and laid the foundation for potential application of Hypsizygus marmoreus as source of nutraceuticals and functional food products. PRACTICAL APPLICATION: A systematic assessment of the potential differences of phenolics, antioxidant, and antiproliferative activities between different Hypsizygus marmoreus varieties was carried out in the present study. Furthermore, our findings would present possible antiproliferative mechanism of extracts of different Hypsizygus marmoreus varieties, which may provide theoretical basis for further development and utilization of Hypsizygus marmoreus.

中文翻译：

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两种海棠品种酚类、抗氧化和抗增殖活性的比较

比较了棕色 Hypsizygus marmoreus (brown HM) 和白色 Hypsizygus marmoreus (white HM) 的酚类物质、抗氧化活性和抗增殖特性。结果表明，棕色HM的(+)-儿茶素、没食子酸和原儿茶酸含量高于白色HM。此外，棕色HM比白色HM具有更高的细胞抗氧化活性（CAA）、过氧自由基清除能力（PSC）和氧自由基吸收能力（ORAC）值，这表明棕色HM具有更强的抗氧化能力。棕色 HM 和白色 HM 均对 HepG2 细胞显示出显着的抗增殖活性，并且棕色 HM 被证明更有效。流式细胞术结果显示，棕色 HM 和白色 HM 均可以剂量依赖性方式诱导 G1 期阻滞和细胞凋亡。此外，CyclinD1、CDK4 和 Bcl-2 mRNA 表达水平随着棕色 HM 或白色 HM 的处理而下调。综上所述，我们的研究表明，棕色 HM 比白色 HM 具有更好的抗氧化和抗增殖活性，并为 Hypsizygus marmoreus 作为营养食品和功能性食品的潜在应用奠定了基础。实际应用：本研究对不同 Hypsizygus marmoreus 品种之间酚类物质、抗氧化和抗增殖活性的潜在差异进行了系统评估。此外，我们的研究结果还揭示了不同品种海参提取物的抗增殖作用机制，为进一步开发利用海参的进一步开发利用提供了理论依据。和 Bcl-2 mRNA 表达水平随着棕色 HM 或白色 HM 的处理而下调。综上所述，我们的研究表明，棕色 HM 比白色 HM 具有更好的抗氧化和抗增殖活性，并为 Hypsizygus marmoreus 作为营养食品和功能性食品的潜在应用奠定了基础。实际应用：本研究对不同 Hypsizygus marmoreus 品种之间酚类物质、抗氧化和抗增殖活性的潜在差异进行了系统评估。此外，我们的研究结果还揭示了不同品种海参提取物的抗增殖作用机制，为进一步开发利用海参的进一步开发利用提供了理论依据。和 Bcl-2 mRNA 表达水平随着棕色 HM 或白色 HM 的处理而下调。综上所述，我们的研究表明，棕色 HM 比白色 HM 具有更好的抗氧化和抗增殖活性，并为 Hypsizygus marmoreus 作为营养食品和功能性食品的潜在应用奠定了基础。实际应用：本研究对不同 Hypsizygus marmoreus 品种之间酚类物质、抗氧化和抗增殖活性的潜在差异进行了系统评估。此外，我们的研究结果还揭示了不同品种海参提取物的抗增殖作用机制，为进一步开发利用海参的进一步开发利用提供了理论依据。