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Procedures for Flow Cytometry-Based Sorting of Unfixed Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infected Cells and Other Infectious Agents.
Cytometry Part A ( IF 3.7 ) Pub Date : 2020-06-03 , DOI: 10.1002/cyto.a.24040
Kristen M Reifel 1 , Brandon K Swan 1 , Evan R Jellison 2 , David Ambrozak 3 , Jan Baijer 4 , Richard Nguyen 3 , Simon Monard 5 , Geoffrey Lyon 6 , Benjamin Fontes 7 , Stephen P Perfetto 3
Affiliation  

In response to the recent COVID‐19 pandemic, many laboratories are involved in research supporting SARS‐CoV‐2 vaccine development and clinical trials. Flow cytometry laboratories will be responsible for a large part of this effort by sorting unfixed antigen‐specific lymphocytes. Therefore, it is critical and timely that we have an understanding of risk assessment and established procedures of infectious cell sorting. Here we present procedures covering the biosafety aspects of sorting unfixed SARS‐CoV‐2‐infected cells and other infectious agents of similar risk level. These procedures follow the ISAC Biosafety Committee guidelines and were recently approved by the National Institutes of Health Institutional Biosafety Committee for sorting SARS‐CoV‐2‐infected cells. © 2020 International Society for Advancement of Cytometry

中文翻译:

未固定的严重急性呼吸系统综合症冠状病毒2(SARS-CoV-2)感染的细胞和其他感染因子的基于流式细胞术排序的程序。

为了应对最近的COVID-19大流行,许多实验室参与了支持SARS-CoV-2疫苗开发和临床试验的研究。流式细胞仪实验室将通过分选未固定的抗原特异性淋巴细胞来负责这项工作。因此,了解风险评估和建立传染性细胞分选的程序至关重要且及时。在这里,我们介绍的程序涵盖了对未固定的SARS-CoV-2感染细胞和具有类似风险水平的其他感染因子进行分类的生物安全方面的程序。这些程序遵循ISAC生物安全委员会的指导原则,并且最近获得了美国国立卫生研究院机构生物安全委员会的批准,可用于对SARS-CoV-2感染的细胞进行分选。©2020国际细胞计数学会
更新日期:2020-07-22
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