Abstract A new green microalga isolate Micractinium sp. GA001 was found to accumulate long-chain fatty acids, and the strain was subjected to flow cytometry-based adaptive evolution approach to produce improved phenotypes. At first, original phenotype of new isolate GA001 was well characterized followed by establishment of flow cytometry conditions in combination with fluorescent dyes BODIPY and Nile Red, to screen intracellular long-chain fatty acids in GA001. Fluorescent dyes staining and flow cytometry analysis revealed the progressive accumulation of desirable lipid components in GA001. Further, a flow cytometry-based strategy was used to selectively isolate and enrich particular GA001 phenotypes with higher accumulation of long-chain fatty acids, under nitrogen-depletion and –repletion conditions. This strategy yielded an improved population with high lipid content than original population. Micractinium sp. GA001 was proved to be a promising strain with improved phenotypes for the production of large-scale target-specific long-chain fatty acids.

中文翻译：

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长链脂肪酸的菌株改良产生 Micractinium sp。通过流式细胞术

摘要 一种新的绿色微藻分离物 Micractinium sp。GA001 被发现积累长链脂肪酸，该菌株经过基于流式细胞术的适应性进化方法以产生改进的表型。首先，对新分离株GA001的原始表型进行了很好的表征，随后建立了流式细胞仪条件，结合荧光染料BODIPY和尼罗红，筛选GA001中的细胞内长链脂肪酸。荧光染料染色和流式细胞术分析揭示了 GA001 中所需脂质成分的逐渐积累。此外，基于流式细胞术的策略用于在氮耗竭和氮耗竭条件下选择性分离和富集长链脂肪酸积累较高的特定 GA001 表型。该策略产生了比原始种群具有高脂质含量的改良种群。Micratinium sp。GA001 被证明是一种具有改进表型的有前途的菌株，可用于生产大规模的目标特异性长链脂肪酸。