The present study was conducted to explore the potentialities of a versatile multifarious plant growth promoting rhizobacteria Bacillus altitudinis MS16 (GenBank Accession no. MG066459) and its effectiveness in phytopathogen inhibition. B.altitudinis MS16 was evaluated in vitro and it was found to fix 82.80 ± 0.6 μg N ml⁻¹ of culture media, can produce 40.74 ± 0.7 μg ml⁻¹ of IAA, release 7.72 ± 0.9 μg ml⁻¹ of insoluble phosphorus and can secrete hydrolytic enzymes. Apart from potassium and zinc solubilization, it exhibited robust motility and biofilm forming abilities. The strain exhibits remarkable root colonizing efficiency and act strongly against phytopathogens like Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, Corynespora cassiicola, Fusarium verticillioides and Fusarium oxysporum f.sp. Pisi with maximum inhibition against S. sclerotiorum (83.0 %) under in-vitro conditions. Since the strain showed promising activity against S. sclerotiorum (ITCC 4042), ultramicroscopic studies were undertaken to investigate their interaction pattern. Ultramicroscopic observations involving SEM, CLSM and AFM revealed severely altered morphology of MS16 treated S. sclerotiorum mycelia. Leaf detached bioassay accompanied with in planta studies showed potential bio-control efficiency of MS16 with 95.7 ± 2.5 % inhibition obtained in leaf detached assay, 96.5 ± 1.7 % and 98.32 ± 0.56 % inhibition in stem and leaf inoculation against S. sclerotiorum. Furthermore, root colonization resulted in prominent root architectural changes. Seed bacterization showed increase in plant growth attributes over the control under in-vitro and in planta conditions. Therefore, the overall results demonstrate the beneficial role of MS16 for growth promotion in mustard plants while controlling sclerotinia infection in mustard.

进行本研究以探究促进根际细菌枯草芽孢杆菌MS16（GenBank登录号MG066459）的多功能杂种植物生长的潜力及其在抑制植物病原体中的有效性。在体外对B.altitudinis MS16进行了评估，发现它固定了82.80± 0.6μgN ml ^-1的培养基，可产生40.74±0.7μgml ^-1的IAA，释放7.72±0.9μgml ^-1的不溶磷和可以分泌水解酶。除了钾和锌的增溶作用外，它还具有强大的运动能力和生物膜形成能力。该菌株显示出显着的根部定植效率，并且对植物病原体如炭疽病，菌核病，黄瓜褐斑病菌，镰刀菌和尖孢镰刀菌病菌 皮丝用针对最大抑制核盘菌下（83.0％）的体外条件。由于该菌株显示出对核盘菌的有希望的活性（ITCC 4042），因此进行了超显微研究以研究它们的相互作用模式。涉及SEM，CLSM和AFM的超显微观察表明，MS16处理的葡萄球菌菌丝体的形态发生了严重变化。与植物分离的叶片分离生物测定法研究表明，MS16具有潜在的生物防治效率，在叶片脱离试验中抑制率为95.7±2.5％，在茎叶接种中对菌核病的抑制率为96.5±1.7％和98.32±0.56％。此外，根部定植导致显着的根部结构变化。在体外和植物条件下，种子杀菌显示植物生长特性比对照提高。因此，总体结果证明了MS16在促进芥菜植物生长同时控制芥菜菌核菌感染方面的有益作用。