Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield. The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding. In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411. JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type. Gibberellic acid (GA) treatment analysis suggested that JE0124 was GA-sensitive. Analysis of the frequency distribution of plant height in four F₂ populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited. We used two F₂ populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus (QTL) for reduced height to a 0.85-cM interval on chromosome 2DL. The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis (BSA) and genetic linkage analysis, with logarithm of odds (LOD) scores of 5.34 and 5.78, respectively. Additionally, this QTL accounted for 8.27–8.52% of the variation in the phenotype. The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breeding.

小麦矮小与抗倒伏性密切相关，这会影响收获指数和谷物产量。新的矮化或半矮化基因的发现对矮化小麦育种具有重要意义。在这项研究中，我们确定了由EMS诱导的来自精英品种Jing411的矮麦突变体JE0124。与野生型相比，JE0124的茎强度更高，株高降低了33％。赤霉素（GA）处理分析表明JE0124对GA敏感。由JE0124之间的杂交得到的四个F ₂种群的株高频率分布分析较高的品种农大5181和野生型表明矮化表型在数量上是遗传的。我们使用了来自农大5181和JE0124的倒数杂交的两个F ₂种群和312个个体，以将高度降低的定量性状基因座（QTL）映射到2DL染色体上的0.85-cM区间。通过结合使用基于660K SNP阵列的大体积分离物分析（BSA）和遗传连锁分析完成映射，优势对数（LOD）得分分别为5.34和5.78。此外，这个QTL占表型变异的8.27–8.52％。本研究中的矮化突变体JE0124和2DL染色体上新发现的矮化基因将丰富矮化小麦育种的遗传资源。