Light-emitting diodes (LEDs) are a new light source with low energy consumption and high photoelectric conversion efficiency, and they can satisfy the energy-saving needs of plant culture systems. However, the effects of LED light sources on rice tissue culture and rice seedling cultivation are poorly understood. This study aimed to evaluate the effects of LEDs on the growth of tissue culture plantlets and seedlings of the rice (Oryza sativa L.) cultivar Nipponbare. The best light source for rice tissue culture was different from that for rice seedling cultivation. Blue (B) LED light was the most appropriate light for rice tissue culture. Under a B LED light, the time required for callus proliferation, differentiation and regeneration was the shortest, and the frequency of plantlet initiation, differentiation and regeneration was the highest. A blue:red (B:R)=1:1 LED light facilitated the growth of rice seedlings and produced the highest chlorophyll and carotenoid contents and photosynthetic rates in the rice seedlings. Abundant photosynthetic products were more effectively generated in the rice seedlings under the B:R=1:1 LED and R LED lights than under the B LED light. B LED light is the most appropriate light for rice tissue culture plantlets and can be used as an alternative light source for rice tissue culture, and B:R=1:1 LED light facilitated the cultivation of robust rice seedlings and can be used as the primary light source for rice factory seedling cultivation.

发光二极管（LED）是一种能耗低，光电转换效率高的新型光源，可以满足植物培养系统的节能需求。但是，人们对LED光源对水稻组织培养和水稻幼苗培养的影响知之甚少。这项研究旨在评估LED对水稻（Oryza sativa）组织培养苗和幼苗生长的影响L.）品种Nipponbare。水稻组织培养的最佳光源与水稻幼苗培养的最佳光源不同。蓝色（B）LED灯是用于水稻组织培养的最合适的灯。在B LED灯下，愈伤组织增殖，分化和再生所需的时间最短，而幼苗的萌发，分化和再生的频率最高。蓝色：红色（B：R）= 1：1的LED灯促进了水稻幼苗的生长，并在水稻幼苗中产生了最高的叶绿素和类胡萝卜素含量以及光合速率。在B：R = 1：1 LED和R LED光照下，水稻幼苗比B LED光照下能更有效地产生丰富的光合产物。