Abstract

The aim of the work was a comparative description of the reaction of cellular elements of venous blood of a person in vitro upon contact with the carbon sorbent used in the small-volume hemoperfusion (SVH) procedure and chitosan fibers. The formation of chitosan fibers was carried out from a 4% solution of chitosan in a 2% solution of acetic acid in a laboratory setup using the coagulation method. Within 60 min of the experiment in samples of blood in contact with hemosorbents in a rotational mode, 26 parameters were recorded on a SySmex XT 1800i hematology analyzer. The optical density was recorded on a UNICO 280 (S) spectrophotometer at wavelengths corresponding to the absorption maxima of the hemoglobin molecule (414 and 540 nm). An analysis of the results showed that chitosan fibers have greater hemocompatibility and the ability to modify the surface of the fibers with selective agents. Based on the data obtained, it can be concluded that chitosan fibers can be used in the SVH procedure both independently and as a neutral matrix for constructing specific hemoactivators for the practical use of this biopolymer in effective medical treatment regimens.

中文翻译：

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人体静脉血中的细胞元素与碳吸血剂和壳聚糖纤维接触后反应的比较特征

摘要

这项工作的目的是对人静脉血中的细胞成分与小剂量血液灌注（SVH）程序中使用的碳吸附剂和壳聚糖纤维接触后体外反应的比较描述。壳聚糖纤维的形成是在实验室设置中使用凝结方法从4％的壳聚糖溶液在2％的乙酸溶液中进行的。在实验的60分钟内，以旋转方式与吸血剂接触的血液样本在SySmex XT 1800i血液分析仪上记录了26个参数。在UNICO 280（S）分光光度计上以对应于血红蛋白分子的最大吸收（414和540nm）的波长记录光密度。结果分析表明，壳聚糖纤维具有更大的血液相容性，并具有使用选择剂修饰纤维表面的能力。根据获得的数据，可以得出结论，壳聚糖纤维既可以独立使用，也可以作为中性基质用于SVH程序，以构建特定的血液活化剂，以在生物医学上有效地用于治疗方案。