Background

Rice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed.

Results

In this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi3 were as the donor parent to establish two BC₃F₁ and derived BC₃F₂ backcross population in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% in 2 years. And, among Jin23B/QingGuAi3 population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% in 2 years.

Conclusions

Sixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi3.

中文翻译：

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基于两个水稻先进回交群体的抗病性QTL鉴定。

背景

稻瘟病是水稻在经济上重要且易变的疾病。使用宿主抗性基因育种抗性品种已被证明是控制稻瘟病最有效，最经济的方法，因此需要新的抗性基因或数量性状基因座（QTL）。

结果

在这项研究中，我们构建了两个先进的回交种群来定位抗病性QTL。CR071和QingGuAi3作为供体，在Jin23B背景中建立了两个BC ₃ F ₁和衍生的BC ₃ F ₂回交种群。通过挑战2011年和2012年这两个自然感染的种群，分别在Jin23B / CR071和Jin23B / QingGuAi3种群中鉴定出16和13个抗爆炸QTL。在Jin23B / CR071人群中，有3个主要QTL和13个次要QTL解释了表型在2年内从3.50％变为34.08％。并且，在Jin23B / QingGuAi3人群中，有2个主要QTL和11个次要QTL解释了2年中表型变异从2.42％到28.95％。

结论

在Jin23B / CR071和Jin23B / QingGuAi3群体中分别鉴定出16和13个抗爆炸QTL。QTL效应分析表明，主要和次要QTL相互作用是水稻CR071和QingGuAi3持久抗稻瘟病的遗传基础。