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Characterization and fine mapping of a leaf yellowing mutant in common wheat
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2020-06-03 , DOI: 10.1007/s10725-020-00633-0
Heng Zhang , Yiming Chen , Ying Niu , Xu Zhang , Jia Zhao , Li Sun , Haiyan Wang , Jin Xiao , Xiue Wang

Creation of leaf color mutants is the options in the study of photosynthesis, chloroplast development and Chlorophyll biosynthesis. In this study, a leaf color mutant NAU31 was identified from tissue culture of common wheat. In general, the mutant exhibited whole plant yellowing, fewer tillering, dwarfing and interestingly, improved resistance to wheat powdery mildew. Photosynthetic physiological analysis showed that the chloroplast inner structure varied in the mesophyll cells of the mutant, and this may lead to reduced chlorophyll content (chlorophyll “a”, Chlorophyll “b”, total chlorophyll and carotenoid), as well as decreased maximum quantum efficiency of photosystem II (Fv/Fm) and net photosynthetic rate. Transcriptome analysis indicated that the impairing of photosynthesis may be responsible for plant yellowing of NAU31. Inheritance analysis showed that NAU31 was a gain-of-function mutant and the yellowing trait was controlled by a dominant gene, namely Taymg (yellow mutant gene). SSR markers analysis preliminarily mapped Taymg to the distal short arm of chromosome 2D using an F2 population from the cross between NAU31 and W7984, a synthesized wheat. The mapping result was confirmed by BSA using a 55 K wheat SNP array. Through recombinants screening, the Taymg was finely mapped to the terminal region of 2DS, flanked by markers ZH47 and gwm296 and corresponding to a physical interval of 0.76 Mb. The cloning of Taymg will facilitate the dissection of the mechanism involved in chloroplast development of wheat and the coordinating of plant development and disease resistance.

中文翻译:

普通小麦叶片黄化突变体的表征与精细定位

叶色突变体的创建是光合作用、叶绿体发育和叶绿素生物合成研究中的选项。在这项研究中,从普通小麦的组织培养中鉴定了一个叶色突变体 NAU31。总的来说,该突变体表现出整株黄化、更少的分蘖、矮化和有趣的是,对小麦白粉病的抗性提高。光合生理分析表明,突变体叶肉细胞的叶绿体内部结构发生变化,这可能导致叶绿素含量(叶绿素“a”、叶绿素“b”、总叶绿素和类胡萝卜素)降低,以及最大量子效率降低光系统 II (Fv/Fm) 和净光合速​​率。转录组分析表明光合作用受损可能是NAU31植物黄化的原因。遗传分析表明,NAU31是功能获得性突变体,黄化性状受显性基因Taymg(黄色突变基因)控制。SSR 标记分析使用 NAU31 和 W7984(一种合成小麦)杂交的 F2 种群初步将 Taymg 定位到染色体 2D 的远端短臂。BSA 使用 55 K 小麦 SNP 阵列确认了映射结果。通过重组筛选,Taymg 精确定位到 2DS 的末端区域,两侧是标记 ZH47 和 gwm296,对应的物理间隔为 0.76 Mb。Taymg的克隆将有助于剖析小麦叶绿体发育的机制以及植物发育与抗病性的协调。
更新日期:2020-06-03
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