The objective of the study is to ascertain the biological potential and to identify the bioactive compounds of the four crude extract hexane, chloroform, ethyl acetate, and methanol from the roots of Houttuynia cordata. The chemical compounds of the extracts have analyzed by Gas Chromatography-Mass Spectrometry (GC–MS) and Thin Layer Chromatography. The biological potential of H. cordata extract was investigated by an antibacterial assay using the disc diffusion method. The four crude extracts of H. cordata, examined on five test microorganisms Escherichia coli, Streptococcus pneumoniae, Klepsilla pneumoniae, Shigella dysentriae, and Bacillus subtilis at a consistency of 1 mg/ml in the study. The outcomes illuminated that ethyl-acetate extract and chloroform extract exhibited significant activity than those of methanol and hexane extract. The GC–MS analysis revealed the presence of sixteen bioactive compounds, three of them are novel (14-Bromo-2-methyltetradec-1-en -3-ol, 6-[4-Hydroxymethyl-4-methylpentyl amino] purine, and Cholest-5-en-3-ol (3Beta), propanoate) and their biological attributes are not yet proclaimed. The effects of the investigation exhibited the presence of the bioactive components in the odorous herb possesses antibacterial activity and may find a place in the pharmaceutical industry as an element of antibiotics.

中文翻译：

---

使用TLC和GC-MS分析对鱼腥草的生物潜力和化学成分进行初步筛选

该研究的目的是确定鱼腥草根的生物潜力并鉴定四种粗提取物己烷，氯仿，乙酸乙酯和甲醇的生物活性化合物 。 提取物的化学成分已通过气相色谱-质谱（GC-MS）和薄层色谱法进行了分析。 使用圆盘扩散法通过抗菌测定研究了堇菜提取物的生物潜力 。 在五个测试微生物大肠杆菌，肺炎链球菌，肺炎克雷伯氏菌，痢疾志贺氏菌和枯草芽孢杆菌的五个测试微生物上检查了 堇青菜的四种粗提物 在研究中浓度为1 mg / ml。结果表明，乙酸乙酯提取物和氯仿提取物比甲醇和己烷提取物显示出显着的活性。GC-MS分析表明存在十六种生物活性化合物，其中三种是新颖的（14-溴-2-甲基十四--1-烯-3-醇，6- [4-羟基甲基-4-甲基戊基氨基]嘌呤和Cholest-5-en-3-ol（3Beta），丙酸酯）及其生物学特性尚未公布。研究的结果表明，在有气味的草药中存在生物活性成分，具有抗菌活性，并可能在制药工业中作为抗生素的一种成分。