Although both estrogen deficiency and diabetes contribute to periodontal tissue deterioration, the combined effects of these conditions on periodontium is unknown. Thus, we analyzed the combined effects of ovariectomy followed by streptozotocin (STZ)-induced diabetes on periodontal tissues of rats. Twenty adult rats were ovariectomized (OVX) or SHAM-operated (SHAM). After 3 weeks, the rats received an intraperitoneal injection of STZ (60 mg/kg/body weight) to induce diabetes or vehicle (blank) solution. The groups were assigned as follows (n = 5): SHAM-vehicle (SHAM), OVX-vehicle (OVX), SHAM + STZ (SHAM-Di), and OVX + STZ (OVX-Di). Seven weeks post-diabetes induction, the rats were euthanized. Blood samples were collected for glucose measurements and maxillae were processed for paraffin embedding. Sections stained with hematoxylin/eosin, Masson’s trichrome, and picrosirius-red were used for alveolar bone loss and collagen fiber analysis in the lamina propria. Immunohistochemistry was performed for runt-related transcription factor 2 (Runx2), matrix metalloproteinase 9 (MMP-9), and tryptase detection. Alveolar bone loss and fewer collagen fibers were observed in the OVX-Di group, collagen fibers with irregular organization, and MMP-9 immunoreactivity were more evident in diabetic groups, and MMP-9-positive osteoclasts on alveolar bone surface were noticed in all groups. The OVX-Di group showed lower Runx2 immunoreactivity (osteoblast formation marker), and more tryptase-positive cells (mast cell marker) in the alveolar bone marrow. Our results indicate that estrogen depletion, followed by STZ-induced diabetes, promotes periodontal tissue deterioration that is more evident than both interventions applied alone. Furthermore, our results points to a possible participation of bone-derived mast cells in this process.

中文翻译：

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雌激素缺乏后链脲佐菌素诱导的糖尿病对雌性大鼠牙周组织的影响。

尽管雌激素缺乏和糖尿病都会导致牙周组织恶化，但这些情况对牙周组织的综合影响尚不清楚。因此，我们分析了卵巢切除术后链脲佐菌素 (STZ) 诱导的糖尿病对大鼠牙周组织的综合影响。二十只成年大鼠被切除卵巢 (OVX) 或假手术 (SHAM)。3 周后，大鼠接受腹腔注射 STZ（60 毫克/千克/体重）以诱导糖尿病或载体（空白）溶液。这些组分配如下 (n = 5)：SHAM-载体 (SHAM)、OVX-载体 (OVX)、SHAM + STZ (SHAM-Di) 和 OVX + STZ (OVX-Di)。糖尿病诱导后七周，大鼠被安乐死。采集血样用于葡萄糖测量，处理上颌骨用于石蜡包埋。用苏木精/曙红染色的切片，Masson 的三色和苦味天狼星红用于牙槽骨丢失和固有层中的胶原纤维分析。对 runt 相关转录因子 2 (Runx2)、基质金属蛋白酶 9 (MMP-9) 和类胰蛋白酶检测进行了免疫组织化学。OVX-Di组牙槽骨丢失和胶原纤维减少，糖尿病组胶原纤维组织不规则，MMP-9免疫反应更明显，各组牙槽骨表面均有MMP-9阳性破骨细胞. OVX-Di 组显示较低的 Runx2 免疫反应性（成骨细胞形成标志物）和肺泡骨髓中更多的类胰蛋白酶阳性细胞（肥大细胞标志物）。我们的结果表明雌激素耗竭，随后是 STZ 诱导的糖尿病，促进牙周组织恶化，这比单独应用两种干预措施更明显。此外，我们的结果表明骨源性肥大细胞可能参与了这一过程。