The application of organophosphorus pesticides (OPPs) increased gradually because of the rise in global food demand that triggered the agriculture sector to increase the production, leading to OPP residues in the surface water. This study elucidated the presence of OPPs and estimated its ecological risk in the riverine ecosystem of the urbanised Linggi River, Negeri Sembilan, Malaysia. The OPP concentration in surface water was determined using solid-phase extraction method and high-performance liquid chromatography coupled with diode array detection. Further, the ecological risk was estimated by using the risk quotient (RQ) method. The three OPPs, i.e. chlorpyrifos, diazinon, and quinalphos were detected with mean concentrations of 0.0275 µg/L, 0.0328 µg/L, and 0.0362 µg/L, respectively. The OPPs were at high risk (in general and worst cases) under acute exposure. The estimated risk of diazinon was observed as medium for general (RQ_m = 0.5857) and high for worst cases (RQ_ex = 4.4678). Notably, the estimated risk for chlorpyrifos was high for both general and worst cases (RQ_m = 1.9643 and RQ_ex = 11.5643) towards the aquatic ecosystem of the Linggi River. Chronic risk of quinalphos remains unknown because of the absence of toxicity endpoints. This study presented clear knowledge regarding OPP contamination and possible risk for aquatic ecosystems. Hence, OPPs should be listed as one of the main priority contaminants in pesticide mitigation management in the future.

由于全球粮食需求的增加触发了有机农业增加产量，导致有机磷农药（OPPs）的使用逐渐增加，导致地表水中的OPP残留。这项研究阐明了OPP的存在，并估计了马来西亚森美兰州城市化的Linggi河的河流生态系统中的OPP生态风险。使用固相萃取法和高效液相色谱结合二极管阵列检测法测定地表水中的OPP浓度。此外，通过使用风险商（RQ）方法估算了生态风险。检出了三种毒死P，分别是毒死rif，二嗪农和奎那磷，平均浓度分别为0.0275 µg / L，0.0328 µg / L和0.0362 µg / L。在急性暴露下，OPPs处于高风险（一般和最坏情况）。估计的二嗪农风险是一般（RQ）的媒介_m  = 0.5857）和最坏情况下较高（RQ _ex  = 4.4678）。值得注意的是，一般情况和最坏情况下， 毒死rif的估计风险对 临吉河水生生态系统都是很高的（RQ _m = 1.9643，RQ _ex = 11.5643）。由于缺乏毒性终点，喹诺磷的慢性风险仍然未知。这项研究提供了有关OPP污染和水生生态系统可能风险的清晰知识。因此，OPPs应该被列为未来农药减缓管理中​​的主要优先污染物之一。