Background

Coccidia are obligatory intracellular parasites with at least one intestinal phase in their life cycles, being Isospora Schneider, 1881 the main coccidian genus related to the order Passeriformes. However, there is no record of isosporans from the passerine family Tityridae, which is the family of the greenish schiffornis Schiffornis virescens (Lafresnaye, 1838).

Purpose

This study aimed to examine the faeces from a greenish schiffornis S. virescens captured in the Itatiaia National Park, State of Rio de Janeiro, Southeastern Brazil, to determine what coccidian parasites were present.

Methods

Only one specimen of Schiffornis virescens was captured with mist nets. Coccidian oocysts were recovered from the fecal samples by flotation in Sheather’s saturated solution. Morphological observations, line drawings, photomicrographs and measurements were made in optical microscopy and digitally edited. The molecular analysis included the study of the sequence of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene, with phylogenetic reconstructions based on the Neighbor-Joining and Maximum Likelihood analysis.

Results

An Isospora sp. considered as new to science is described and identified from Schiffornis virescens (Lafresnaye, 1838). Isospora oliveirai n. sp. has oocysts that are subspheroidal, 26.0 × 24.8 μm, with rough, bilayered wall, c.2.5 μm thick. Micropyle and oocyst residuum absent, but one to six polar granules are present. Sporocysts lemon-shaped, 18.1 × 10.9 μm. The Stieda body is knob-like to half-moon-shaped and sub-Stieda is rounded. Sporocyst residuum is present, composed of scattered spherules of different sizes. Sporozoites are vermiform, with refractile bodies and nucleus. Molecular analysis at the COI gene exhibited similarity of 97% with Isospora serinuse Yang, Brice, Elliot et Ryan, 2015 from island canaries Serinus canaria (Linnaeus, 1758), and Isospora spp. from great tits Parus major (Linnaeus, 1758) and European robins Erithacus rubecula (Linnaeus, 1758).

Conclusion

Based on the morphological and molecular features, I. oliveirai is considered as new to science and the first coccidian species recorded from Tityridae.

中文翻译：

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Isospora Oliveirai n. sp. (Chromista: Miozoa: Eimeriidae) 来自南美洲的 Greenish Schiffornis Schiffornis virescens (Lafresnaye, 1838) (Passeriformes: Tyranni: Tityridae)。

背景

球虫是必需的细胞内寄生虫，在其生命周期中至少有一个肠道阶段，Isospora Schneider，1881 是与 Passeriformes 目有关的主要球虫属。然而，没有来自雀形目鹟科的同孢菌的记录，该科是绿色的雪佛兰Schiffornis virescens (Lafresnaye, 1838)。

目的

本研究旨在检查在巴西东南部里约热内卢州 Itatiaia 国家公园捕获的绿色 schiffornis S. virescens的粪便，以确定存在哪些球虫寄生虫。

方法

只有一个Schiffornis virescens标本被雾网捕获。通过在希瑟氏饱和溶液中浮选从粪便样品中回收球虫卵囊。形态观察、线条图、显微照片和测量均在光学显微镜下进行并进行数字编辑。分子分析包括研究线粒体细胞色素c氧化酶亚基 1 (COI) 基因的序列，以及基于邻居连接和最大似然分析的系统发育重建。

结果

等孢菌属。从Schiffornis virescens (Lafresnaye, 1838) 中描述和鉴定出被认为是科学新事物。Isospora Oliveirai n. sp. 具有亚球形的卵囊，26.0 × 24.8 μm，具有粗糙的双层壁，c。2.5 微米厚。没有微孔和卵囊残留物，但存在 1 到 6 个极性颗粒。孢子囊柠檬形，18.1 × 10.9 μm。Stieda 体呈旋钮状至半月形，子 Stieda 呈圆形。存在孢子囊残留物，由不同大小的分散小球组成。子孢子是蠕虫状的，具有折射体和细胞核。COI 基因的分子分析显示与Isospora serinuse 的相似性为 97%Yang、Brice、Elliot 和 Ryan，2015 年来自岛屿金丝雀Serinus canaria（Linnaeus，1758）和Isospora spp。来自大山雀Parus Major (Linnaeus, 1758) 和欧洲知更鸟Erithacus rubecula (Linnaeus, 1758)。

结论

根据形态学和分子特征，橄榄球蚴被认为是科学界的新物种，也是第一个从 Tityridae 记录的球虫物种。