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Natural infection potential and efficacy of the entomopathogenic fungus Beauveria bassiana against Orosanga japonica (Melichar)
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2020-06-02 , DOI: 10.1186/s41938-020-00269-2
M. Mustafa Akıner , Murat Öztürk , İbrahim Güney , Asu Usta

In this study, potential of the entomopathogenic fungi (EPF) isolates from dead samples of Orosanga japonica (Melichar) (Hemiptera: Ricaniidae) was evaluated. Infected specimens with fungi results confirmed that the samples were Beauveria bassiana after morphological and molecular identification. Amplicons produced two haplotypes (h = 2), which included one polymorphic site after sequence and named isolates 1 and 2. Two haplotypes were rooted with MK229193.1, MG345084.1 B. bassiana Genbank samples. Two-year survey results of the EPF effects revealed the highest mortality and natural infection rates in the field. Natural mortality rates varied between 80.35% (Fındıklı-August) and 94.8% (Çayeli-September) in 2018 and between 79.82% (Alipaşa-August) and 97.75% (Fındıklı- September) in 2019. For nymphs, the lowest LT50 value was found at 2.92 days for isolate 1 and 2.56 days for isolate 2, with a concentration of 1 × 106 conidia/ml, using the leaf dipping method. For adults, the lowest LT50 value was found at 3.02 days for isolate 1 and 3.15 days for isolate 2, with a concentration of 1 × 106 conidia/ml, using the direct spraying method. Nymph LT50 values were found a little bit lower than adults. Direct spraying methods results gave a high LT50 value for nymph in contrast to adult. Although the LT50 ratios gave high/low degree in different isolates, methods and life stages, non-significant differences were found between each other’s (p > 0.05). In general, B. bassiana natural infection rates were found high in August and September during the 2 years. Efficacy of the two isolates, which derived from naturally infected O. japonica specimens, increased with concentration. The two isolates can potentially be used for O. japonica integrated management, as a fungal biocontrol agent, but their toxicological effects on beneficial insects, such as honeybees, will need to be determined.

中文翻译:

自然感染的潜力和昆虫病原真菌的功效白僵菌Orosanga粳稻(蝉)

在这项研究中,评估了来自日本大叶鸢尾(Melichar)(半翅目:Ricaniidae)的死样品中的昆虫病原真菌(EPF)分离物的潜力。经真菌感染的标本经形态和分子鉴定证实为球孢白僵菌。扩增子产生两个单倍型(h = 2),其在序列后包括一个多态性位点,分别命名为分离物1和2。两个单倍型以MK229193.1,MG345084.1芽孢杆菌Genbank样品为根。EPF效果的两年调查结果显示该领域的死亡率和自然感染率最高。在2018年,自然死亡率在80.35%(Fındıklı-August)和94.8%(Çayeli-September)之间变化,在2019年的79.82%(Alipaşa-August)和97.75%(Fındıklı-9月)之间变化。对于若虫,最低的LT50值是发现于2。使用叶浸法,分离株1为92天,分离株2为2.56天,浓度为1×106分生孢子/ ml。对于成年人,使用直接喷雾法发现分离株1的最低LT50值为3.02天,分离株2的最低LT50值为3.15天,分生孢子浓度为1×106分生孢子/ ml。发现若虫LT50值比成人低。与成人相比,直接喷涂方法的结果为若虫提供了较高的LT50值。尽管LT50比率在不同的分离物,方法和生命阶段中具有高/低程度,但彼此之间没有显着差异(p> 0.05)。通常,两年中八月和九月发现了球孢杆菌的自然感染率很高。来源于自然感染的日本粳稻标本的两种分离物的功效随浓度的增加而增加。
更新日期:2020-06-02
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