Two types of pyridoxal analogs, azido pyridoxal (PL-N₃) and carboxyl pyridoxal (PL-COOH), were developed as novel bifunctional bioorthogonal molecules. These molecules showed fast imine formation with hydrazinyl groups and stable covalent linkages via azido/carboxyl groups, and thus were of great use for site-specific peptide and protein modifications.

中文翻译：

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双功能吡ido醛衍生物可作为生物大分子修饰的有效生物正交试剂。

已开发出两种类型的吡molecules醛类似物，叠氮基吡ido醛（PL-N ₃）和羧基吡ido醛（PL-COOH），作为新型的双功能生物正交分子。这些分子显示具有肼基的快速亚胺形成以及通过叠氮基/羧基的稳定共价键，因此对于位点特异性肽和蛋白质修饰非常有用。