Objectives. To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored. Methods. Flow cytometry is used for cell identification. The differentiation ability of hBM-MSCs was studied by differentiation culture. SA-β-gal staining is used to detect cell senescence levels. Western blot and immunofluorescence were used to determine protein expression levels. RT-qPCR is used to detect mRNA expression levels. Results. Rg1 regulates the differentiation of hBM-MSCs. Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis. Western blot results showed that Rg1 regulated the overactivation of the β-catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3β. GSK-3β inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3β, which in turn reduced Rg1-induced differentiation of hBM-MSCs. Conclusion. Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3β and regulate the mesenchymal stem cell differentiation ability.

中文翻译：

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Ginsenoside Rg1 通过 GSK-3β 和 β-Catenin 抑制人骨髓间充质干细胞的衰老来改善分化。

目标。证明人参皂甙 Rg1 对人骨髓间充质干细胞 (hBM-MSCs) 分化的影响。随后，探索了检测影响间充质干细胞分化的Rg1的合理机制。方法。流式细胞仪用于细胞鉴定。通过分化培养研究hBM-MSCs的分化能力。SA - β -gal 染色用于检测细胞衰老水平。蛋白质印迹和免疫荧光用于确定蛋白质表达水平。RT-qPCR 用于检测 mRNA 表达水平。结果. Rg1 调节 hBM-MSCs 的分化。分化培养分析表明，Rg1促进细胞成骨和软骨形成。Western blot结果显示，Rg1调节了β -catenin信号通路的过度激活，并显着调节了GSK-3β的磷酸化。GSK-3 β抑制剂 (Licl) 显着增加了 Rg1 诱导的 GSK-3 β磷酸化，从而降低了 Rg1 诱导的 hBM-MSCs 分化。结论。人参皂甙 Rg1 可通过抑制 GSK-3 β的磷酸化，减少衰老细胞中 Wnt 通路的过度激活，调节间充质干细胞的分化能力。