Colorectal cancer (CRC) is the fourth leading cause of tumor-related deaths worldwide. In this study, we explored the in vivo effects of quercetin, a plant flavonol from the flavonoid group of polyphenols with antioxidant effects, on colon carcinogenesis induced by azoxymethane/dextran sodium sulfate (AOM/DSS). Thirty mice were randomly assigned into three groups: the control group, the AOM/DSS group, and the quercetin+AOM/DSS group. CRC was induced by AOM injection and a solution of 2% DSS in the drinking water. In the AOM/DSS-induced colon cancer mice model, quercetin treatment dramatically reduced the number and size of colon tumors. In addition, quercetin significantly restored the leukocyte counts by decreasing the inflammation caused by AOM/DSS. We also observed that the expression of oxidative stress markers, such as lipid peroxide (LPO), nitric oxide (NO), superoxide dismutase (SOD), glucose-6-phosphate (G6PD), and glutathione (GSH), could be reduced by quercetin, suggesting that the anti-inflammatory function of quercetin comes from its antioxidant effect. Moreover, potential biomarkers were identified with serum metabolite profiling. Increased levels of 2-hydroxybutyrate, 2-aminobutyrate, and 2-oxobutyrate and decreased levels of gentian violet, indole-3-methyl acetate, N-acetyl-5-hydroxytryptamine, indoxyl sulfate, and indoxyl were also found in the AOM/DSS-treated mice. However, quercetin treatment successfully decreased the levels of 2-hydroxybutyrate, 2-aminobutyrate, 2-oxobutyrate, endocannabinoids, and sphinganine and increased the levels of gentian violet, N-acetyl-5-hydroxytryptamine, indoxyl sulfate, and indoxyl. Together, our data demonstrated that quercetin could maintain relatively potent antitumor activities against colorectal cancer in vivo through its anti-inflammation effect.

中文翻译：

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槲皮素通过抗小鼠炎症作用抑制AOM / DSS诱导的结肠癌发生。

结直肠癌（CRC）是世界范围内与肿瘤相关的死亡的第四大主要原因。在这项研究中，我们探讨了槲皮素（一种来自多酚类黄酮的植物类黄酮，具有抗氧化作用）对由乙氧基甲烷/葡聚糖硫酸钠（AOM / DSS）诱导的结肠癌发生的体内作用。将三十只小鼠随机分为三组：对照组，AOM / DSS组和槲皮素+ AOM / DSS组。通过AOM注射和饮用水中2％DSS溶液诱导CRC。在AOM / DSS诱导的结肠癌小鼠模型中，槲皮素治疗显着减少了结肠肿瘤的数量和大小。此外，槲皮素可通过减少AOM / DSS引起的炎症来显着恢复白细胞计数。我们还观察到氧化应激标记（例如脂质过氧化物（LPO），槲皮素可减少一氧化氮（NO），超氧化物歧化酶（SOD），6-磷酸葡萄糖（G6PD）和谷胱甘肽（GSH）的含量，这表明槲皮素的抗炎作用来自其抗氧化作用。此外，通过血清代谢物谱鉴定了潜在的生物标志物。在AOM / DSS中还发现2-羟基丁酸酯，2-氨基丁酸酯和2-氧代丁酸酯的含量增加，龙胆紫，吲哚-3-乙酸甲酯，N-乙酰基-5-羟基色胺，硫酸吲哚酚和吲哚酚的含量降低。治疗的小鼠。但是，槲皮素治疗成功降低了2-羟基丁酸酯，2-氨基丁酸酯，2-氧代丁酸酯，内源性大麻素和鞘氨醇的含量，并增加了龙胆紫，N-乙酰基-5-羟基色胺，硫酸吲哚酚和吲哚酚的水平。一起，