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Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis.
Disease Markers ( IF 3.464 ) Pub Date : 2020-05-25 , DOI: 10.1155/2020/2509454
Liang Wang 1 , Xiaoqian Shang 1 , Xinwei Qi 1 , Derong Ba 1 , Jie Lv 1 , Xuan Zhou 1 , Hao Wang 2 , Nazierhan Shaxika 2 , Jing Wang 1, 3 , Xiumin Ma 1, 4
Affiliation  

Background. Macrophages are important immune cells involved in Mycobacterium tuberculosis (M.tb) infection. To further investigate the degree of disease development in patients with spinal tuberculosis (TB), we conducted research on macrophage polarization. Methods. Thirty-six patients with spinal TB and twenty-five healthy controls were enrolled in this study. The specific morphology of tuberculous granuloma in spinal tissue was observed by hematoxylin-eosin (H&E) staining. The presence and distribution of bacilli were observed by Ziehl-Neelsen (ZN) staining. Macrophage-specific molecule CD68 was detected by immunohistochemistry (IHC). M1 macrophages play a proinflammatory role, including the specific molecule nitric oxide synthase (iNOS) and the related cytokine tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). M2 macrophages exert anti-inflammatory effects, including the specific molecule CD163 and related cytokine interleukin-10 (IL-10). The above markers were all detected by quantitative real-time PCR (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and IHC. Results. Typical tuberculous granuloma was observed in the HE staining of patients with spinal TB. ZN staining showed positive expression of Ag85B around the caseous necrosis tissue and Langerhans multinucleated giant cells. At the same time, IHC results indicated that CD68, iNOS, CD163, IL-10, TNF-α, and IFN-γ were expressed around the tuberculous granuloma, and their levels were obviously higher in close tissue than in the distant tissue. RT-PCR and ELISA results indicated that IL-10, TNF-α, and IFN-γ levels of TB patients were also higher than those of the healthy controls. Conclusion. The report here highlights that two types of macrophage polarization (M1 and M2) are present in the tissues and peripheral blood of patients with spinal TB. Macrophages also play proinflammatory and anti-inflammatory roles. Macrophage polarization is involved in spinal TB infection.

中文翻译:

脊髓结核患者M1/M2巨噬细胞及相关细胞因子的临床意义。

背景。巨噬细胞是参与结核分枝杆菌( M.tb ) 感染的重要免疫细胞。为了进一步调查脊柱结核 (TB) 患者的疾病发展程度,我们对巨噬细胞极化进行了研究。方法. 36 名脊柱结核患者和 25 名健康对照者参加了这项研究。苏木精-伊红(H&E)染色观察脊柱组织结核性肉芽肿的具体形态。通过 Ziehl-Neelsen (ZN) 染色观察杆菌的存在和分布。通过免疫组织化学 (IHC) 检测巨噬细胞特异性分子 CD68。M1巨噬细胞发挥促炎作用,包括特定分子一氧化氮合酶(iNOS)和相关细胞因子肿瘤坏死因子(TNF - α)和干扰素(IFN- γ ))。M2巨噬细胞发挥抗炎作用,包括特定分子CD163和相关的细胞因子白细胞介素10(IL-10)。上述标志物均通过定量实时PCR(RT-PCR)、酶联免疫吸附试验(ELISA)和IHC检测。结果。脊柱结核患者的HE染色观察到典型的结核性肉芽肿。ZN染色显示干酪样坏死组织和朗格汉斯多核巨细胞周围Ag85B阳性表达。同时,IHC结果表明,CD68、iNOS、CD163、IL-10、TNF- α和IFN- γ在结核性肉芽肿周围表达,且其在近处组织中的含量明显高于远处组织。RT-PCR 和 ELISA 结果表明 IL-10、TNF-结核病患者的α和 IFN- γ水平也高于健康对照组。结论。此处的报告强调,脊髓结核患者的组织和外周血中存在两种类型的巨噬细胞极化(M1 和 M2)。巨噬细胞还发挥促炎和抗炎作用。巨噬细胞极化与脊髓结核感染有关。
更新日期:2020-05-25
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