当前位置: X-MOL 学术bioRxiv. Mol. Biol. › 论文详情
Expression of a Malassezia codon optimized mCherry fluorescent protein in a bicistronic vector
bioRxiv - Molecular Biology Pub Date : 2020-06-01 , DOI: 10.1101/2020.04.16.044297
Joleen P.Z. Goh; Giuseppe Ianiri; Joseph Heitman; Thomas L. Dawson

The use of fluorescent proteins allows a multitude of approaches from live imaging and fixed cells to labelling of whole organisms, making it a foundation of diverse experiments. Tagging a protein of interest or specific cell type allows visualization and studies of cell localization, cellular dynamics, physiology, and structural characteristics. In specific instances fluorescent fusion proteins may not be properly functional as a result of structural changes that hinder protein function, or when overexpressed may be cytotoxic and disrupt normal biological processes. In our study, we describe application of a bicistronic vector incorporating a Picornavirus 2A peptide sequence between a NAT antibiotic selection marker and mCherry. This allows expression of multiple genes from a single open reading frame and production of discrete protein products through a cleavage event within the 2A peptide. We demonstrate integration of this bicistronic vector into a model Malassezia species, the haploid strain M. furfur CBS 14141, with both active selection, high fluorescence, and proven proteolytic cleavage. Potential applications of this technology can include protein functional studies, Malassezia cellular localization, and co-expression of genes required for targeted mutagenesis.
更新日期:2020-06-01

 

全部期刊列表>>
AI核心技术
10years
材料学研究精选
Springer Nature Live 产业与创新线上学术论坛
胸腔和胸部成像专题
自然科研论文编辑服务
ACS ES&T Engineering
ACS ES&T Water
屿渡论文,编辑服务
杨超勇
周一歌
华东师范大学
段炼
清华大学
廖矿标
李远
跟Nature、Science文章学绘图
隐藏1h前已浏览文章
中洪博元
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
x-mol收录
福州大学
南京大学
王杰
左智伟
电子显微学
何凤
洛杉矶分校
吴杰
赵延川
试剂库存
天合科研
down
wechat
bug