Knowledge of factors driving reproductive isolation is essential to understand the process of speciation. To study the reproductive isolation of two closely related species with overlapping flowering seasons, Iris domestica and Iris dichotoma, we compared their reproductive system, floral biology and pollination biology. The results indicated that I. domestica was facultatively xenogamous, whereas I. dichotoma was facultatively autogamous. Although the two species differed significantly in floral colour, floral diameter, floral structure, nectar volume, flower opening and closing times, they shared the same diurnal pollinator, the honeybee Apis cerana. The frequency of pollination by A. cerana did not differ significantly between the two species, but honeybee pollination of I. domestica was more efficient compared with that of I. dichotoma. Despite the difference in floral structure between the two species, both species deposited pollen on the same parts of the body of honeybees. The temporal partitioning of within-day flowering times between I. domestica (from 07.15 to 08.15 h to 18.00 to 19.00 h) and I. dichotoma (from 15.45 to 16.15 h to 22.00 to 23.00 h), together with the time memory of honeybees, meant that the two species did not overlap in the time of their pollination, thus leading to temporal isolation as a major driver of reproductive isolation between the two species.

中文翻译：

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在一天之内临时分离两种鸢尾科（鸢尾科）共享同一传粉媒介的情况

了解驱动生殖隔离的因素对于理解物种形成过程至关重要。为了研究的两个密切相关的物种生殖隔离重叠开花季节，射干和鸢尾萁，我们比较了他们的生殖系统，花卉生物学和传粉生物学。结果表明：一，家蝇是兼xenogamous，而一萁是兼自花受精。尽管这两个物种在花色，花直径，花结构，花蜜量，花开闭时间上有显着差异，但它们共享相同的昼夜授粉媒介，蜜蜂Apis cerana。cer.cerana授粉的频率没有两个物种间显著不同，但蜜蜂授粉一家蝇与相比是更有效的一萁。尽管两个物种的花艺结构有所不同，但两个物种的花粉都沉积在蜜蜂身体的相同部位。的内天开花之间的时间的时间分割I.家蝇（从07.15到08.15小时以18.00至19.00 h）和I.萁（从15.45到16.15小时以22.00至23.00 h）中，连同蜜蜂的时间存储器，意味着这两个物种在授粉时没有重叠，因此导致暂时隔离成为两个物种之间生殖隔离的主要驱动力。