SummaryThe efficiency of in vitro embryo production technologies would be improved by the development of suitable non-invasive biomarkers that allow the selection of good quality cumulus–oocyte complexes (COCs). The present study used whole, single oocyte culture to investigate whether the expression levels of follicle-stimulating hormone receptor (FSHR), insulin-like factor 1 receptor (IGF1R) and three steroidogenesis-related enzymes (CYP11al, CYP19al and HSD3β) in cumulus cells reflected the developmental competence of COCs. Cumulus cells were collected from single COCs before maturation culture and relative mRNA levels were assessed using real-time PCR. The analysis indicated that mRNAs for FSHR, IGF1R, CYP11al and HSD3β were present at higher levels in cumulus cells from COCs that failed to form blastocysts compared with cumulus cells from COCs that formed blastocysts. Moreover, FSHR and IGF1R mRNA levels were positively correlated with those of genes for steroidogenesis-related enzymes. In conclusion, poor developmental competence of COCs was related to higher expression of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells, which may indicate the advanced differentiation of cumulus cells into granulosa cells.

中文翻译：

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FSHR、IGF1R、CYP11al和HSD3β在卵丘细胞中的表达水平可以预测牛卵母细胞的体外发育能力

总结效率体外胚胎生产技术将通过开发合适的非侵入性生物标志物来改进，这些生物标志物允许选择高质量的卵丘-卵母细胞复合物 (COC)。本研究使用完整的单卵母细胞培养来研究卵丘细胞中促卵泡激素受体 (FSHR)、胰岛素样因子 1 受体 (IGF1R) 和三种类固醇生成相关酶 (CYP11al、CYP19al 和 HSD3β) 的表达水平。反映了 COC 的发展能力。在成熟培养之前从单个 COC 收集卵丘细胞，并使用实时 PCR 评估相对 mRNA 水平。分析表明，mRNAFSHR,IGF1R,CYP11al和HSD3β与来自形成囊胚的 COC 的卵丘细胞相比，来自未能形成囊胚的 COC 的卵丘细胞中的含量更高。而且，FSHR和IGF1RmRNA水平与类固醇生成相关酶的基因水平呈正相关。总之，COCs 发育能力差与FSHR,IGF1R,CYP11al和HSD3β在卵丘细胞中，这可能表明卵丘细胞向颗粒细胞的高级分化。