SummaryThe objective of the present study was to elucidate whether resveratrol could facilitate the survival of boar sperm during liquid preservation and fast cooling processes. Boar semen were diluted with Modena extender containing different concentrations of resveratrol. Sperm motility was evaluated by visual estimation. Membrane integrity, acrosome integrity and mitochondrial membrane potentials were measured by SYBR-14/PI, FITC-PNA and JC-1 staining, respectively. Moreover, the levels of reactive oxygen species (ROS), malonaldehyde (MDA) and total antioxidant capacity (T-AOC) were measured using commercial assay kits. B-cell lymphoma protein-2 (BCL2) content was determined by western blotting. During liquid preservation at 17oC, the addition of 50 μM resveratrol to the Modena extender significantly improved sperm motility, membrane integrity, acrosome integrity, and sperm mitochondrial membrane potentials. Similar results were also observed in the 150 μM resveratrol group during the fast cooling process. Furthermore, addition of resveratrol led to a decrease of ROS and MDA, and an increase in the content of T-AOC and BCL2. These observations suggest that addition of resveratrol to Modena extender protects boar sperm against oxidative stress. The optimal concentrations of resveratrol are 50 μM and 150 μM during liquid preservation and fast cooling process, respectively.

中文翻译：

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白藜芦醇通过其抗氧化能力在体外保护公猪精子

摘要本研究的目的是阐明白藜芦醇在液体保存和快速冷却过程中是否可以促进公猪精子的存活。用含有不同浓度白藜芦醇的Modena稀释剂稀释公猪精液。通过视觉估计评估精子活力。膜完整性、顶体完整性和线粒体膜电位分别通过 SYBR-14/PI、FITC-PNA 和 JC-1 染色测量。此外，使用商业化验试剂盒测量活性氧（ROS）、丙二醛（MDA）和总抗氧化能力（T-AOC）的水平。通过蛋白质印迹测定 B 细胞淋巴瘤蛋白 2 (BCL2) 含量。在 17 液体保存期间○C，在 Modena 稀释剂中添加 50 μM 白藜芦醇可显着改善精子活力、膜完整性、顶体完整性和精子线粒体膜电位。在快速冷却过程中，在 150 μM 白藜芦醇组中也观察到了类似的结果。此外，添加白藜芦醇导致ROS和MDA减少，T-AOC和BCL2含量增加。这些观察结果表明，在 Modena 补充剂中添加白藜芦醇可以保护公猪精子免受氧化应激。在液体保存和快速冷却过程中，白藜芦醇的最佳浓度分别为 50 μM 和 150 μM。