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Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nature Biotechnology ( IF 46.9 ) Pub Date : 2020-06-01 , DOI: 10.1038/s41587-020-0509-0
Rina C Sakata 1, 2 , Soh Ishiguro 1, 3, 4 , Hideto Mori 1, 3, 4 , Mamoru Tanaka 1 , Kenji Tatsuno 5 , Hiroki Ueda 6 , Shogo Yamamoto 5 , Motoaki Seki 1 , Nanami Masuyama 1, 3, 4 , Keiji Nishida 7, 8 , Hiroshi Nishimasu 9 , Kazuharu Arakawa 3, 4, 10 , Akihiko Kondo 7, 8, 11 , Osamu Nureki 9 , Masaru Tomita 1, 3, 4, 10 , Hiroyuki Aburatani 5 , Nozomu Yachie 1, 2, 3, 4, 9, 12
Affiliation  

We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.



中文翻译:

同时引入C-to-T和A-to-G突变的基础编辑器。

我们描述了结合了胞嘧啶和腺嘌呤碱基编辑功能的碱基编辑器。胞嘧啶脱氨酶PmCDA1,腺苷脱氨酶TadA和Cas9切口酶(Target-ACEmax)的密码子优化融合显示了47个基因组靶点同时具有较高的同时C-T和A-G编辑活性。Target-ACEmax的靶向活性以及DNA和RNA脱靶活性与现有的单功能碱基编辑器相似。

更新日期:2020-06-01
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