Abstract Atrazine is the most common herbicide applied in crops of economic relevance, such as sugar cane, soybean, and corn. Atrazine and its derivatives desethylatrazine (DEA) and desisopropylatrazine (DIA) are toxic to the environment, affecting animal and human health. Thus, this study aimed to evaluate the degradation of atrazine and its derivatives by the fungus Pleurotus ostreatus INCQS 40310, as well as the potential of the enzymes involved in this process. P. ostreatus INCQS 40310 was able to degrade atrazine (82%), DEA (71%), and DIA (56%) over 22 days of fungal cultivation. Proteomic analysis indicated the participation of hydrolases and peroxidases during the degradation process. Additionally, resting cells of the fungus were tested to verify the action of intracellular enzymes in the degradation process, suggesting the participation of cytochrome P450 enzymatic complex. Resting cells experiments promoted the degradation of 50% of atrazine, 36% of DIA, 30% of DEA. So far, this is the first work evaluating the biodegradation of DEA and DIA by fungus. Graphical Abstract

中文翻译：

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阿特拉津、去乙基莠去津 (DEA) 和去异丙基莠去津 (DIA) 被平菇 INCQS 40310 降解

摘要 阿特拉津是最常见的经济作物除草剂，如甘蔗、大豆和玉米。阿特拉津及其衍生物去乙基莠去津 (DEA) 和去异丙基莠去津 (DIA) 对环境有毒，影响动物和人类健康。因此，本研究旨在评估真菌 Pleurotus ostreatus INCQS 40310 对莠去津及其衍生物的降解，以及参与该过程的酶的潜力。P. ostreatus INCQS 40310 能够在 22 天的真菌培养中降解莠去津 (82%)、DEA (71%) 和 DIA (56%)。蛋白质组学分析表明水解酶和过氧化物酶在降解过程中的参与。此外，还测试了真菌的静息细胞，以验证细胞内酶在降解过程中的作用，表明细胞色素 P450 酶复合物的参与。静息细胞实验促进了 50% 的莠去津、36% 的 DIA、30% 的 DEA 的降解。迄今为止，这是第一项评估真菌对 DEA 和 DIA 生物降解的工作。图形概要