The main goal of this study was to assess the effect of different sterilization treatment for sterilization of decellularized kidney tissue. Rabbit kidneys were decellularized by the perfusion-based method using sodium dodecyl sulfate (SDS) and Triton X-100. Then, decellularized kidney slices were prepared and sterilized by an antibiotic cocktail, PAA (0.5 %, 1% and 1.5 %), 5KG γ-irradiation and 320–480 nm UV-irradiation. Histological evaluations, DNA quantification assay, MTT assay, scanning electron microscopy (SEM), mechanical test and bacterial and fungal culture tests were performed to determine the quality of decellularization and sterilization processes. The kidney slices were seeded by adipose-derived mesenchymal stem cells (ASCs) to assess the cell adhesion capability after treatment. The results of the current study indicated that PAA 0.5 % was the most efficient method to completely decontaminate rabbit decellularized kidney tissue while preserving the mechanical properties and main components of the matrix which are necessary for cell-matrix interaction and cell adhesion. The 5KG γ-irradiation was determined to be the most destructive sterilization method, with reduced the mechanical strengths as well as altered microstructure of the kidney matrix and no cell adhesion. In addition, UV-irradiation is not able to sterile the decellularized tissues. Therefore PAA 0.5 % sterilization method can be a powerful means for sterilization of biological scaffolds.

这项研究的主要目的是评估不同的灭菌处理对脱细胞肾脏组织灭菌的效果。使用十二烷基硫酸钠（SDS）和Triton X-100通过基于灌注的方法使兔肾脏脱细胞。然后，准备去细胞的肾脏切片，并用抗生素鸡尾酒，PAA（0.5％，1％和1.5％），5KGγ射线和320-480 nm紫外线射线灭菌。进行组织学评估，DNA定量测定，MTT测定，扫描电子显微镜（SEM），机械测试以及细菌和真菌培养测试，以确定脱细胞和灭菌过程的质量。用脂肪来源的间充质干细胞（ASC）播种肾脏切片，以评估治疗后的细胞粘附能力。当前研究的结果表明PAA为0。5％是完全净化兔去细胞肾组织的最有效方法，同时保留了机械特性和基质对细胞-基质相互作用和细胞粘附所必需的主要成分。确定5KGγ射线是最具破坏性的灭菌方法，其机械强度降低，肾脏基质的微观结构发生改变，并且没有细胞粘附。另外，紫外线照射不能使脱细胞的组织无菌。因此，PAA 0.5％灭菌方法可以成为对生物支架灭菌的有力手段。