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Biocatalytic production of isoamyl acetate from fusel oil in supercritical CO2
The Journal of Supercritical Fluids ( IF 3.9 ) Pub Date : 2020-06-02 , DOI: 10.1016/j.supflu.2020.104917
Arthur Luiz Baião Dias , Tahmasb Hatami , Julian Martínez , Ozan N. Ciftci

Isoamyl acetate esters were enzymatically synthesized from fusel oil, a by-product generated in bioethanol industrial plants, in supercritical carbon dioxide (SC-CO2) using immobilized lipase (Lipozyme 435). Effects of enzyme load (2.5–10.0 %), substrates’ molar ratio (acetic anhydride to fusel oil; 1:1–4:1), acyl donor (acetic anhydride, ethyl acetate, and acetic acid) and solvent type (SC-CO2, n-hexane, and solvent-free system) on the biocatalytic isoamyl acetate conversion and the specific productivity were investigated. Higher volumetric expansions of the reaction medium were observed at higher temperature (60 °C) between 10 and 20 MPa. A monophasic system was observed at 15 MPa and 60 °C. Acetic anhydride and SC-CO2 were the best acyl donor and solvent, respectively. An elementary mathematical model was adjusted to the experimental data. 15 MPa, 60 °C, 2:1 substrates’ molar ratio and 2.5 % of enzyme concentration yielded the highest conversion (88.4 ± 0.5 %) and specific productivity (36.9 ± 0.2 %).



中文翻译:

超临界CO 2中杂醇油的生物催化生产乙酸异戊酯

使用固定化脂肪酶(Lipozyme 435),在超临界二氧化碳(SC-CO 2)中,由杂醇油(生物乙醇工业工厂产生的副产物)酶促合成乙酸异戊酯。酶负荷(2.5–10.0%),底物摩尔比(乙酸酐与杂醇油; 1:1–4:1),酰基给体(乙酸酐,乙酸乙酯和乙酸)和溶剂类型(SC- CO 2ñ在生物催化乙酸异戊酯的转化率和比生产率-己烷,和无溶剂系统)进行了调查。在更高的温度(60°C)和10到20 MPa之间观察到反应介质的更高的体积膨胀。在15 MPa和60°C下观察到单相系统。乙酸酐和SC-CO 2分别是最好的酰基供体和溶剂。将基本数学模型调整为实验数据。在15 MPa,60°C,2:1底物的摩尔比和2.5%的酶浓度下,转化率最高(88.4±0.5%),比生产率最高(36.9±0.2%)。

更新日期:2020-06-02
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