The mechanism underlying the effect of bioelectric field on wound healing in vivo has not been previously investigated. Here, we aimed to investigate the effects of an applied electric field (EF) on epidermal cell migration during wound healing. Using a Bama miniature pig wound model, we applied a power-up device (negative electrode in the wound centre and positive electrode around the wound) with pulsed electrical power, to apply a continuous, stable, and tolerable EF to the wound and provide directional signals for keratinocyte migration towards the wound centre. An EF of 100 mV/mm applied in the same direction as the bioelectric field accelerated wound healing. The keratinocytes exhibited regular and similar shapes, uniform arrangement, and an organised migration pattern. In contrast, 100 mV/mm applied countercurrent to the bioelectric field, delayed wound healing and hindered the keratinocyte migration towards the wound centre. Further, the cells were disorganised, misshapen, irregular, and disoriented. Via the application of a directional stable EF, this study morphologically identified the relationships among wound EF, keratinocyte migration, and wound healing and established theoretical and empirical foundations for the clinical application of bioelectric fields.

以前尚未研究过生物电场对体内伤口愈合的潜在作用机理。在这里，我们旨在研究伤口愈合过程中施加电场（EF）对表皮细胞迁移的影响。使用Bama微型猪伤口模型，我们使用了带有脉冲电源的加电装置（伤口中心处的负电极和伤口周围的正电极），以对伤口施加连续，稳定且可耐受的EF并提供定向角质形成细胞向伤口中心迁移的信号。在与生物电场相同的方向上施加100 mV / mm的EF可以加速伤口愈合。角质形成细胞显示规则和相似的形状，均匀的排列和有组织的迁移模式。相反，将100 mV / mm的电流反向施加到生物电场，延迟伤口愈合并阻碍角质形成细胞向伤口中心迁移。此外，细胞杂乱无章，畸形，不规则和不定向。通过定向稳定的EF的应用，本研究从形态学上确定了伤口EF，角质形成细胞迁移和伤口愈合之间的关系，并为生物电场的临床应用奠定了理论和经验基础。