Abstract

The effect of short-term salt stress induced by carbonate salinization on the indicators of oxidative stress, the activity of antioxidant enzymes, and the content of some metabolites in winter triticale seedlings were studied. The experiment was carried out on 7-day-old plants after 12, 24, 48, 72, and 96 h of exposure to 120 mM sodium carbonate solution. The content of malonic dialdehyde, hydrogen peroxide, and the activity of enzymes (catalase, guaiacol peroxidase, and ascorbate peroxidase) were determined. It was shown that carbonate salinization causes significant changes in all mentioned parameters. Thus, more than a threefold increase in the content of hydrogen peroxide was noted in triticale shoots already after 12 h of the experiment. Intensified lipid peroxidation was also observed in the shoots during the first days of salinization. Less significant increase in both indicators was recorded in the roots. An increased activity of the studied enzymes (ascorbate peroxidase, guaiacol peroxidase, and catalase) was noted in the shoots. The analysis of the results showed that the specificity of carbonate salinization can be attributed to more intense accumulation of hydrogen peroxide in the shoots compared with the roots and a decrease in MDA content in triticale shoots by 48 h of exposure.

中文翻译：

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小黑麦幼苗中碳酸盐盐化诱导的氧化胁迫

摘要

研究了碳酸盐盐碱化引起的短期盐胁迫对冬小麦黑小麦幼苗氧化应激指标，抗氧化酶活性以及某些代谢产物含量的影响。在暴露于120 mM碳酸钠溶液中12、24、48、72和96小时后，对7天大的植物进行了实验。测定丙二醛，过氧化氢的含量以及酶（过氧化氢酶，愈创木酚过氧化物酶和抗坏血酸过氧化物酶）的活性。结果表明，碳酸盐盐化会引起所有上述参数的显着变化。因此，在实验12小时后，小黑麦芽中过氧化氢的含量增加了三倍以上。在盐渍化的最初几天，还观察到芽中脂质过氧化加剧。根部记录到这两个指标的增加幅度均较小。在芽中注意到所研究的酶（抗坏血酸过氧化物酶，愈创木酚过氧化物酶和过氧化氢酶）活性增加。结果分析表明，碳酸盐盐化的特异性可以归因于与根相比，芽中过氧化氢的积累更强烈，而暴露于黑小麦的芽中48 h的MDA含量降低。