Abstract

The effect of the surface of Ti-Zr-Nb (TZN) shape memory alloys (SMA) on adhesion, proliferation, viability, and organization of the actin cytoskeleton of osteoblast-like MG-63 cells has been studied. The studied SMA have a unique combination of mechanical properties, making them promising in fabrication of bone implants with high biomechanical compatibility (due to low Young’s modulus and superelastic behavior similar to that of bone tissue). Thin sheets of the experimental TZN alloy and Ti-Al-Nb (TAN) medical alloy (as a control) are used in the study. Growth dynamics of MG-63 cell culture is assessed by the MTT assay and counting the number of nuclei per unit area. It is found that the number of cells cultured on TZN alloy is higher than on TAN alloy on the fourth and seventh days. This may be due to the influence of the qualitative and quantitative composition of the materials on the microstructure and chemistry of the surface. The cell viability during the cell culturing on both alloys is close to 100%. Image-based actin cytoskeleton analysis has shown the predominance of fibrillar actin on the TZN samples and has also revealed the cytoskeletal organization or structure typical of polygonal-shaped fibroblast-like cells.

中文翻译：

---

Ti-Zr-Nb形状记忆合金表面类似于成骨细胞的MG-63细胞的体外相互作用的特征

摘要

研究了Ti-Zr-Nb（TZN）形状记忆合金（SMA）的表面对成骨样MG-63细胞粘附，增殖，生存力和肌动蛋白细胞骨架组织的影响。所研究的SMA具有独特的机械性能组合，使其在具有高生物力学相容性的骨植入物的制造中很有前途（由于较低的杨氏模量和与骨组织相似的超弹性行为）。研究中使用了实验性TZN合金和Ti-Al-Nb（TAN）医用合金的薄板（作为对照）。MG-63细胞培养物的生长动力学通过MTT分析评估，并计算每单位面积的核数。发现在第4天和第7天，在TZN合金上培养的细胞数量高于在TAN合金上培养的细胞数量。这可能是由于材料的定性和定量组成对表面的微观结构和化学性质的影响。在两种合金上进行细胞培养时的细胞活力接近100％。基于图像的肌动蛋白细胞骨架分析显示了在TZN样品上原纤维肌动蛋白的优势，并且还揭示了典型的多边形成纤维细胞样细胞的细胞骨架组织或结构。