Abstract

The capacity decidualization was examined of cultured cells derived from healthy women and patients with gynecological pathology . The decidualization was induced by combination of estradiol with progesterone and its new analogues. The technique of isolation, cultivation of endometrial cell lines, as well as cell morphology, immunophenotype, karyotype, expression of progesterone and estrogen receptors are described. It was shown that cell lines isolated from the endometrium of gynecologically healthy donors and exposed to combinations of estradiol with progesterone and its analogues underwent decidual transformation. The cell lines established from endometrial tissue of patients diagnosed with endometriosis and cell lines isolated from endometrial tissue with hyperplastic processes were insensitive to these hormones. Our results confirm the available data on disordered decidualization processes in patients with endometriosis or endometrial hyperplasia and are a prerequisite for the development of personalized gestagen therapy for gynecological diseases.

中文翻译：

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子宫内膜细胞系在规范和病理条件下的蜕膜分化

摘要

容量蜕皮变性检查了来自健康妇女和妇科病理患者的培养细胞。雌二醇与孕酮及其新类似物的结合导致蜕膜化。描述了分离，培养子宫内膜细胞系的技术，以及细胞形态，免疫表型，核型，孕激素表达和雌激素受体的技术。结果表明，从妇科健康捐献者的子宫内膜分离的细胞系经历了蜕膜转化，暴露于雌二醇与孕酮及其类似物的组合。从诊断为子宫内膜异位的患者的子宫内膜组织建立的细胞系和从具有增生过程的子宫内膜组织分离的细胞系对这些激素不敏感。