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Characteristics of Bone Marrow Progenitor Cells of C57BL/6N Mice after 30-Day Hindlimb Suspension and 12-Hour Readaptation to Support Loading
Cell and Tissue Biology Pub Date : 2020-04-13 , DOI: 10.1134/s1990519x20020078
E. A. Markina , P. I. Bobyleva , O. Yu. Alekseeva , I. V. Andrianova , E. R. Andreeva , L. B. Buravkova

Abstract

The functional status of bone marrow (BM) progenitor cells, which provide hematopoiesis and homeostasis of bone tissue, determines many processes which take a place in spaceflight (SF) and subsequent recovery to gravity. It is known that SF factors and simulation of their effects do irreversible damage to the pool of hematopoietic and stromal progenitor cells. Meanwhile, changes in the functional activity of BM progenitor cells after simulation of SF effects were more pronounced than in the case of a real SF. The aim of the study was to examine processes of recovery of the functional activity of BM progenitor cells during readaptaion to loading after 30-day hindlimb suspension. Studies were performed on three groups of male mice: vivarium control, suspension, and readaptation. The following characteristics were monitored: number of nucleated BM cells, immunophenotype, proliferative activity of stromal BM cells, number of hematopoietic colony-forming units (CFU), CFUs of fibroblasts, and differentiating capabilities of BM hematopoietic and stromal precursors. Hindlimb suspension resulted in decreased number of fibroblast and hematopoietic CFU, inhibition of spontaneous osteodifferentiation of stromal progenitors and decreased expression of stromal markers. The readaptaion period was accompanied with restoration of the functional activity of stromal progenitors, while complete recovery of hematopoiesis was not observed.


中文翻译:

后肢悬吊30天和12小时再适应以支持负荷后C57BL / 6N小鼠的骨髓祖细胞的特征

摘要

骨髓(BM)祖细胞的功能状态提供造血作用和骨骼组织的稳态,决定了航天(SF)中发生的许多过程以及随后的重力恢复。众所周知,SF因子及其作用的模拟确实对造血和基质祖细胞池造成不可逆的损害。同时,在模拟SF效应之后,BM祖细胞的功能活性的变化比真实SF的情况更为明显。该研究的目的是研究后肢悬吊30天后再适应负荷过程中BM祖细胞功能活性的恢复过程。对三组雄性小鼠进行了研究:饲养箱对照,悬浮和重新适应。监视以下特征:有核BM细胞的数量,免疫表型,基质BM细胞的增殖活性,造血集落形成单位(CFU)的数量,成纤维细胞的CFU以及BM造血和基质前体的分化能力。后肢悬吊导致成纤维细胞和造血CFU数量减少,基质祖细胞自发性骨分化抑制和基质标志物表达降低。重新适应期伴随着基质祖细胞功能活性的恢复,而未观察到造血功能的完全恢复。后肢悬吊导致成纤维细胞和造血CFU数量减少,基质祖细胞自发性骨分化抑制和基质标志物表达降低。重新适应期伴随着基质祖细胞功能活性的恢复,而未观察到造血功能的完全恢复。后肢悬吊导致成纤维细胞和造血CFU数量减少,基质祖细胞自发性骨分化抑制和基质标志物表达降低。重新适应期伴随着基质祖细胞功能活性的恢复,而未观察到造血功能的完全恢复。
更新日期:2020-04-13
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