Abstract

The aim of this study was to determine the genetic variation of Colored horses by using microsatellite markers. In the study, samples from a total of 28 Colored horse were genotyped using 17 microsatellite markers, 12 of them are recommended by the International Society for Animal Genetics (ISAG) and the other 5 loci included by the StockMarks Genotyping Kit. A total of 129 different alleles were identified and the average number of alleles was 7.588.The heterozygosity value (H_o) was 0.286 as the lowest (LEX3) and 0.929 (HTG10) as the highest among the markers; the expected lowest heterozygosity value (H_e) was determined as 0.607 (HTG4) and the highest one was 0.876 (VHL20). The mean H_o and H_e values were calculated as 0.729 and 0.771, respectively. On a population basis, the mean F_IS and F_IT values were 0.047. The mean PIC value was 0.741. In the phylogenetic tree formed by the neighbour joining tree (NJT), it was determined that the horses were generally categorized into 3 groups. Although statistically significant results (P = 0.01008) were found in the studies investigating whether the population of Colored horses had the risk of extinction or not, it was determined that the data showed normal L distribution indicating no risk of recent extinction. It was determined that the Colored horses had a genetically important potential.

中文翻译：

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利用微卫星标记对土耳其有色马进行遗传变异的研究

摘要

这项研究的目的是通过使用微卫星标记来确定有色马的遗传变异。在这项研究中，使用17种微卫星标记对来自28匹有色马的样本进行了基因分型，其中12种是国际动物遗传学会（ISAG）推荐的，StockMarks基因分型试剂盒中包括了其他5个基因座。共鉴定出129个等位基因，平均等位基因数为7.588。杂合度值（H _o）最低（LEX3）为0.286，最高为0.929（HTG10）。预期最低杂合度值（H _e）确定为0.607（HTG4），最高杂合度值（HHL）为0.876（VHL20）。均值H _o和H _e值分别计算为0.729和0.771。以人口为基础，平均F _IS和F _IT值为0.047。PIC平均值为0.741。在由邻居结合树（NJT）形成的系统发育树中，已确定马通常分为3组。尽管在调查有色马种群是否具有灭绝风险的研究中发现了统计学上显着的结果（P = 0.01008），但可以确定该数据显示正常的L分布表明没有近期灭绝的风险。已经确定，有色马具有遗传上的重要潜力。