Abstract

For the first time, relative expression levels of the ToxA effector gene and PtrPf2 transcription factor gene in vitro were used to characterize the collection of isolates of the causative agent of tan spot of wheat, Pyrenophora tritici-repentis, from geographically distant populations. It was demonstrated that two groups of isolates, from Kazakhstan (Almaty oblast) and Russia (Krasnodar krai), differed in the levels of genetic diversity. The group of Krasnodar isolates was more heterogenous in expression levels of both genes. In Krasnodar isolates, positive correlation between the expression levels of examined genes was revealed (r = 0.96, p < 0.001). The isolate groups were statistically significantly different in the PtrPf2 expression levels (p = 0.002). According to preliminary data, high virulence of Kazakhstan isolates was associated with high expression levels of the PtrPf2 transcription factor gene in these isolates. A supposition on the existence of association between the PtrPf2 expression level in vitro and the ability of isolates to induce necrosis on the leaves of a susceptible cultivar is made.

中文翻译：

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体外培养的ToxA和PtrPf2基因表达中的小麦支原体分离株之间的差异

摘要

对于第一次，的相对表达水平的ToxA效应基因和PtrPf2转录因子基因在体外进行了表征小麦，棕褐色点的病原体的分离物的收集核腔小麦-repentis，从地理上远离的人口。结果表明，来自哈萨克斯坦（阿拉木图州）和俄罗斯（克拉斯诺达尔边疆区）的两组分离株的遗传多样性水平不同。克拉斯诺达尔分离株的组在两个基因的表达水平上更加异质。在克拉斯诺达尔分离物中，揭示了所检查基因表达水平之间的正相关性（r = 0.96，p<0.001）。PtrPf2表达水平的分离株在统计学上有显着差异（p = 0.002）。根据初步数据，哈萨克斯坦分离株的高毒力与这些分离株中PtrPf2转录因子基因的高表达水平有关。假设在体外PtrPf2表达水平与分离株在易感品种的叶片上诱导坏死的能力之间存在关联。