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The Administration of Semax and HLDF-6 Peptides to Rats Regulates Protein Synthesis Rhythm in Hepatocytes and Corrects Senescent Disturbances
Russian Journal of Developmental Biology ( IF 0.7 ) Pub Date : 2020-03-01 , DOI: 10.1134/s1062360420020034
V. Y. Brodsky , Y. A. Zolotarev , L. A. Malchenko , L. A. Andreeva , D. S. Lazarev , N. N. Butorina , V. S. Kozik , N. F. Myasoedov

Abstract To clarify the organizing effect of Semax and HLDF-6 peptides on the kinetics of protein synthesis in hepatocytes, in addition to an in vitro study (Brodsky et al., 2019), the effects of these peptides in vivo have been studied. The circahoralian (ultradian) rhythm of protein synthesis, that is, a marker of the direct cell-to-cell communication, was investigated in rats of different ages. Peptides were injected intraperitoneally into young (2–3-month-old) or old (1.5–2-year-old) rats at a 50–100-µg/kg dose. Hepatocytes were isolated and sparse or dense cultures were established. In sparse cultures from young rats that received one or another peptide, the rhythm of protein synthesis was observed; in the cultures from rats of the same age that were injected with saline, no rhythm was found. In dense cultures of old rats after the action of the peptide, the amplitudes of the rhythm of protein synthesis did not differ from the rhythms observed in young rats; after saline injection, the rhythm amplitudes were twice as low. Injection of the peptide into adult rats that had previously received dopamine caused a protein synthesis rhythm characteristic for rats of this age; the administration of dopamine abolished the rhythm. The synchronizing effect of the peptides was maintained for at least 2 days after their single administration to the rat. The use of Semax or HLDF-6 is recommended to compensate for the disturbances of the kinetics of protein synthesis in humans in aging and pathology.

中文翻译:

Semax 和 HLDF-6 肽对大鼠的给药调节肝细胞中的蛋白质合成节律并纠正衰老障碍

摘要 为了阐明 Semax 和 HLDF-6 肽对肝细胞蛋白质合成动力学的组织作用,除了一项体外研究 (Brodsky et al., 2019) 外,还研究了这些肽在体内的作用。在不同年龄的大鼠中研究了蛋白质合成的昼夜节律 (ultradian),即细胞间直接通讯的标志物。将肽以 50-100-μg/kg 的剂量腹膜内注射到年轻(2-3 个月大)或年长(1.5-2 岁)大鼠体内。分离肝细胞并建立稀疏或密集的培养物。在接受一种或另一种肽的年轻大鼠的稀疏培养物中,观察到蛋白质合成的节律;在注射生理盐水的同龄大鼠的培养物中,没有发现节律。在肽作用后的老年大鼠的密集培养物中,蛋白质合成节律的幅度与在年轻大鼠中观察到的节律没有区别;注射生理盐水后,节律幅度降低了两倍。将肽注射到先前接受过多巴胺的成年大鼠体内会导致该年龄大鼠的蛋白质合成节律特征;多巴胺的管理废除了节律。在对大鼠单次给药后,肽的同步作用至少维持 2 天。建议使用 Semax 或 HLDF-6 来补偿人类衰老和病理学中蛋白质合成动力学的干扰。节律振幅低了两倍。将肽注射到先前接受过多巴胺的成年大鼠体内会导致该年龄大鼠的蛋白质合成节律特征;多巴胺的管理废除了节律。在对大鼠单次给药后,肽的同步作用至少维持 2 天。建议使用 Semax 或 HLDF-6 来补偿人类衰老和病理学中蛋白质合成动力学的干扰。节律幅度低了两倍。将肽注射到先前接受过多巴胺的成年大鼠体内会导致该年龄大鼠的蛋白质合成节律特征;多巴胺的管理废除了节律。在对大鼠单次给药后,肽的同步作用至少维持 2 天。建议使用 Semax 或 HLDF-6 来补偿人类衰老和病理学中蛋白质合成动力学的干扰。在对大鼠单次给药后,肽的同步作用至少维持 2 天。建议使用 Semax 或 HLDF-6 来补偿人类衰老和病理学中蛋白质合成动力学的干扰。在对大鼠单次给药后,肽的同步作用至少维持 2 天。建议使用 Semax 或 HLDF-6 来补偿人类衰老和病理学中蛋白质合成动力学的干扰。
更新日期:2020-03-01
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