Abstract

The extraction of polyphenol component of arctic brown algae of the Fucus vesiculosus type and the subsequent fractionation of the extract by column chromatography on a Sephadex LH-20 adsorbent are carried out. The obtained fractions are characterized by size-exclusion chromatography, HPLC with UV and mass spectrometric detection (HPLC–UV-MS), and matrix-assisted laser desorption/ionization mass spectrometry (MALDI–MS). Using the MALDI–MS method, the presence of oligomeric compounds containing up to 13 phloroglucin structural units is detected. Using HPLC–UV-MS, oligomeric compounds in the mass range 374–870 Da (trimers–octamers) are detected, and their ratio is estimated.

中文翻译：

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液相色谱-质谱法研究水榕属北极褐藻提取物中的多酚成分

摘要

进行了褐藻（Fusus vesiculosus）型北极褐藻中多酚成分的提取以及随后在Sephadex LH-20吸附剂上的柱色谱分离。所获得的馏分通过尺寸排阻色谱，带紫外线和质谱检测的HPLC（HPLC-UV-MS）以及基质辅助激光解吸/电离质谱（MALDI-MS）进行表征。使用MALDI – MS方法，可以检测到含有多达13个间苯三酚结构单元的寡聚化合物的存在。使用HPLC-UV-MS，可检测质量范围为374-870 Da（三聚体-八聚体）的低聚化合物，并估算其比率。