Abstract

In the present study, an immunosorbent surface-based theophylline determination method was developed and validated. The procedure was tested on milk samples. Theophylline was removed from milk by an immunosorbent surface. The efficiency of theophylline removal was found to be between 95–97%. The proposed method is cost effective and does not require any special equipment. Detection and quantification limits of the proposed method were determined based on the standard deviation of 10 ng/mL theophylline and they were equal 3 and 11 ng/mL, respectively. Results were also validated for precision, accuracy and linearity.

中文翻译：

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经验证的基于免疫吸附剂的牛奶中茶碱的含量测定

摘要

在本研究中，开发并验证了一种基于表面免疫吸附剂的茶碱测定方法。该程序在牛奶样品上进行了测试。通过免疫吸附表面从牛奶中去除茶碱。发现茶碱的去除率在95–97％之间。所提出的方法具有成本效益，并且不需要任何特殊设备。基于10 ng / mL茶碱的标准偏差确定了该方法的检测限和定量限，分别等于3和11 ng / mL。还对结果的准确性，准确性和线性进行了验证。