Abstract

Electrochemical properties of regorafenib (REG) were studied in 0.1 M Britton−Robinson buffer-methanol solutions (3 : 2, v/v) with pH between 3 and 8 at carbon paste electrode by cyclic and differential pulse voltammetry. The results exhibited irreversible anodic peak at about 0.90 V vs. Ag/AgCl, NaCl (3 M). The anodic peak was found to be diffusion–adsorption controlled. Mechanism of REG electrochemical reaction was studied by performing density functional theory computations and mass spectrometric analysis. A validated differential pulse voltammetry (DPV) technique for REG determination was performed. The calibration curve of REG on carbon paste electrode was linear in the concentration range of 0.5–13 μg/mL and limit of detection was 0.10 µg/mL. The recommended DPV method was used to detect REG in spiked plasma and urine specimens and average recoveries were 94%.

中文翻译：

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碳糊电极微分脉冲伏安法测定抗癌药物瑞戈非尼：电化学研究和密度泛函理论计算

摘要

通过循环和微分脉冲伏安法，在碳糊电极的pH为3至8的0.1 M Britton-Robinson缓冲液-甲醇溶液（3：2，v / v）中研究了regorafenib（REG）的电化学性质。结果在相对于Ag / AgCl，NaCl（3M）的约0.90V处显示出不可逆的阳极峰。发现阳极峰受扩散-吸附控制。通过进行密度泛函理论计算和质谱分析研究了REG电化学反应的机理。进行了一种有效的用于REG测定的差分脉冲伏安法（DPV）技术。碳糊电极上REG的校准曲线在0.5-13μg/ mL的浓度范围内是线性的，检出限为0.10μg/ mL。