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Compound droplet dynamics of a tumor cell squeezing through conical microfilters
Theoretical and Computational Fluid Dynamics ( IF 3.4 ) Pub Date : 2020-06-01 , DOI: 10.1007/s00162-020-00534-y
Pengliang Chang , Christopher Landry , Xiaolin Chen , Hua Tan

Circulating tumor cells (CTCs) are regarded as important biomarkers for early cancer detection and treatment. Decades of research have made progress in CTC detection using deformability-based microfilters; however, developing a high-throughput CTC microfilter remains a challenging task due to the lack of the essential understanding of microscopic multiphase flow. To design and optimize a CTC microfilter, in-depth studies of the dynamics of a CTC squeezing through a confined constriction are necessary. In this study, numerical simulation was employed. Utilizing the octree-based Adaptive-Mesh-Refinement algorithm, a CTC was modeled as a compound Newtonian droplet moving through a microfilter with non-uniform cross sections. The immiscible interface was tracked by the volume-of-fluid method with the surface tension accounted for using the continuum surface force method. Pressure signature, shear stress and instantaneous cell velocity during the passing process through a conical microfilter were investigated in great detail in order to understand the fluid dynamics affecting the cell squeezing process. Then, the crucial design parameters including pore angles and operating flow rates were analyzed. The shear stress and critical pressure under different flow rates were investigated as well. Results reveal that the deformation-induced surface tension pressure of the cell nucleus is the dominant component of the critical pressure. Additionally, the maximum instantaneous cell velocity, shear stress and pressure all occur at the same critical stage, as the nucleus passes through the exit of the microfilter channel. Our study provides insights into the dynamics of a compound droplet squeezing through a conical-shaped microfilter and offers constructive guidance for the design and optimization of high-throughput CTC microfilters.

中文翻译:

通过锥形微过滤器挤压肿瘤细胞的复合液滴动力学

循环肿瘤细胞(CTC)被认为是早期癌症检测和治疗的重要生物标志物。数十年的研究在使用基于变形能力的微过滤器检测 CTC 方面取得了进展;然而,由于缺乏对微观多相流的基本了解,开发高通量 CTC 微过滤器仍然是一项具有挑战性的任务。为了设计和优化 CTC 微过滤器,需要深入研究 CTC 通过受限收缩挤压的动力学。在这项研究中,采用了数值模拟。利用基于八叉树的自适应网格细化算法,CTC 被建模为复合牛顿液滴移动通过具有非均匀横截面的微过滤器。不混溶界面通过流体体积法跟踪,表面张力使用连续表面力法计算。对通过锥形微过滤器的过程中的压力特征、剪切应力和瞬时细胞速度进行了非常详细的研究,以了解影响细胞挤压过程的流体动力学。然后,分析了包括孔角和操作流速在内的关键设计参数。还研究了不同流速下的剪切应力和临界压力。结果表明,细胞核的变形诱导表面张力压力是临界压力的主要组成部分。此外,最大瞬时细胞速度、剪切应力和压力都发生在同一临界阶段,当细胞核通过微过滤器通道的出口时。我们的研究提供了对通过锥形微过滤器挤压的复合液滴动力学的见解,并为高通量 CTC 微过滤器的设计和优化提供了建设性的指导。
更新日期:2020-06-01
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