Abstract In the present study, silver nanoparticles (AgNPs) were synthesized using Prunus avium gum extract and their physicochemical properties together with antibacterial and cytotoxic effects were evaluated. The characterization of these AgNPs was performed by UV–vis spectroscopy, transmission electron microscopy, scanning electron microscopy, and Fourier transforms infrared spectroscopy. The bio-performance of the nanoparticles was also evaluated. Our results exhibit the prominent antibacterial activity of AgNPs against both Gram-positive and Gram-negative bacteria. The MTT results indicate cytotoxicity of AgNPs on MCF-7 and SH-SY5Y cell lines at 50 and 10 µg mL −1 concentrations, respectively. Moreover, gene expression analysis by real-time PCR represents the upregulation of Bax mRNA and downregulation of Bcl-2 mRNA, demonstrates the induction of apoptosis after AgNPs treatment. These findings imply that green-synthesized AgNPs could be a promising candidate in biomedical applications such as battling cancer and infectious diseases. Graphic abstract The scheme of preparation of AgNPs and its effect on cell and bacteria.

中文翻译：

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天然树胶作为生物还原剂绿色合成银纳米颗粒：评估 MCF-7 和 SH-SY5Y 细胞系的凋亡功效及其抗菌潜力

摘要 在本研究中，银纳米粒子 (AgNPs) 使用李属鸟胶提取物合成，并评估了它们的理化性质以及抗菌和细胞毒性作用。这些 AgNPs 的表征是通过紫外-可见光谱、透射电子显微镜、扫描电子显微镜和傅里叶变换红外光谱进行的。还评估了纳米颗粒的生物性能。我们的结果显示了 AgNPs 对革兰氏阳性和革兰氏阴性细菌的显着抗菌活性。MTT 结果表明 AgNPs 对 MCF-7 和 SH-SY5Y 细胞系的细胞毒性分别为 50 和 10 µg mL -1 浓度。此外，实时 PCR 的基因表达分析代表 Bax mRNA 的上调和 Bcl-2 mRNA 的下调，证明了在 AgNPs 处理后诱导细胞凋亡。这些发现意味着绿色合成的 AgNPs 可能是生物医学应用（如抗击癌症和传染病）中的一个有希望的候选者。图形摘要 AgNPs 的制备方案及其对细胞和细菌的影响。