当前位置: X-MOL 学术Plant Mol. Biol. Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Phylogenetic and Expression Analyses of Cullin Family Members Unveil the Role of PbCUL1.C1 in Pollen Tube Growth Underlying Non-self S-RNase in Pear
Plant Molecular Biology Reporter ( IF 2.1 ) Pub Date : 2020-06-01 , DOI: 10.1007/s11105-020-01221-2
Ye Zhou , Yongdan Huang , Lei Wu , Guoming Wang , Chao Gu , Shaoling Zhang

Cullin ( CUL ) gene family organizes the largest class of RING E3 ligases which play crucial roles in the ubiquitin-proteasome system to affect plant physiological activities. However, little is known about the CUL genes in fruit trees. In this study, 57 CUL genes were isolated from the sequenced genomes of seven Rosaceae trees and were divided into three subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that multiple duplication events play important role in the expansion of the CUL gene family in Rosaceae . The expression pattern shows that only four CUL genes were highly expressed in pollen and pollen tube in pear. Of these CUL genes, PbCUL1.C1 presented the highest levels of expression in pollen, but the knockdown of PbCUL1.C1 by antisense oligonucleotide (ASO) did not influence pollen tube growth. Interestingly, the pollen tube treated by both the non-self S-RNase and ASO of PbCUL1.C1 was longer than that treated by unique non-self S-RNase, suggesting that the PbCUL1.C1 plays an important role for pollen tube growth underlying non-self S-RNase in pear. These results will be useful for exploring the biological roles of CUL genes in self-incompatibility in Rosaceae trees.

中文翻译:

Cullin 家族成员的系统发育和表达分析揭示了 PbCUL1.C1 在梨非自身 S-RNase 花粉管生长中的作用

Cullin (CUL) 基因家族组织了最大的一类 RING E3 连接酶,它们在泛素-蛋白酶体系统中发挥关键作用,影响植物生理活动。然而,人们对果树中的 CUL 基因知之甚少。在这项研究中,从七株蔷薇科树的测序基因组中分离出 57 个 CUL 基因,并根据系统发育分析将其分为三个亚科。进化分析表明,多次重复事件在蔷薇科CUL基因家族的扩增中起重要作用。表达模式表明,梨花粉和花粉管中只有4个CUL基因高表达。在这些 CUL 基因中,PbCUL1.C1 在花粉中的表达水平最高,但反义寡核苷酸 (ASO) 对 PbCUL1.C1 的敲低不会影响花粉管的生长。有趣的是,PbCUL1.C1 的非自体 S-RNase 和 ASO 处理的花粉管比独特的非自体 S-RNase 处理的花粉管长,表明 PbCUL1.C1 在非自体的花粉管生长中起重要作用梨中的自身 S-RNase。这些结果将有助于探索 CUL 基因在蔷薇科树木自交不亲和中的生物学作用。
更新日期:2020-06-01
down
wechat
bug