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Chrysanthemum is a new host of a group 16SrIII phytoplasma (16SrIII-X) that induces colour-breaking in affected plants
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2020-06-01 , DOI: 10.1007/s10658-020-02027-4
Felipe Franco de Oliveira , Jacson Ferreira , Sarah Rodrigues Galvão , Elliot Watanabe Kitajima , Ivan Paulo Bedendo

Chrysanthemum plants showing symptoms typically induced by phytoplasmas, characterized by colour-breaking and virescence, were found with high incidences in commercial fields. Based on these symptoms, the present investigation aimed to confirm the association of phytoplasmas with affected plants. Expected genomic fragments of 1.2 Kb were generated by nested PCR revealing the occurrence of phytoplasma in all diseased plants. The presence of phytoplasma was confirmed by transmission electron microscopy that detected pleomorphic cells in the inner of phloem vessels. Nucleotide sequences from Chrysanthemum colour-breaking phytoplasma shared 99% similarity with the reference phytoplasma of the 16SrIII-X subgroup. The perfect identity was confirmed by computer-simulated RFLP analyses and similarity coefficient calculations (F equal to 1.0), as well as by the phylogenetic tree, which showed that both phytoplasmas emerge from the same branch. Our findings revealed for the first time the occurrence of a representative of the 16SrIII-X subgroup in Brazil. In addition, it also revealed that Chrysanthemum represents a novel host of a phytoplasma of the 16SrIII group in the world, since, to our knowledge, representatives of this group have not been reported inducing diseases in plants from this genus.



中文翻译:

菊花是16SrIII植物原质体(16SrIII-X)的新宿主,它能在受影响的植物中诱导变色

在商业领域中发现具有典型由植物质浆诱导的症状,特征在于颜色分解和绿化的菊花植物。基于这些症状,本研究旨在确认植物原虫与受影响植物的关联。巢式PCR产生了预期的1.2 Kb基因组片段,揭示了所有患病植物中的植物原质。通过透射电子显微镜证实了植物浆体的存在,该显微镜检测了韧皮部血管内部的多形细胞。菊花的核苷酸序列破色植物质体与16SrIII-X亚组的参照植物质体具有99%的相似性。通过计算机模拟的RFLP分析和相似系数计算(F等于1.0)以及系统发育树确认了完美的同一性,这表明两种植物质体均来自同一分支。我们的发现首次揭示了巴西16SrIII-X亚组的代表。此外,它还揭示了菊花代表了世界上16SrIII组植物质体的新型宿主,因为据我们所知,尚未报道该组的代表在该属植物中诱发疾病。

更新日期:2020-06-01
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