Abstract This study was carried out to evaluate the effect of the various gamma dose irradiations on the antioxidant activity of rosemary PBS (phosphate-buffered saline) extract. The PBS extract of rosemary was irradiated with gamma rays at the doses of 10, 20, 30, 40 and 50 kGy, and their antioxidant activities were investigated by DPPH (1,1-diphenyl-2-picrylhydrazyl), radical cation ABTS•+ (2,2-Azino-Bis (3-Ethylbenzthiazoline-6-Sulfonic Acid), Diammonium Salt), ferric reducing antioxidant power assay (FRAP) and ferric chelating capacity using ferrozine. The scavenging activities of gamma irradiated rosemary were observed to be significantly higher at 30 and 40 kGy using both methods, DPPH and ABST. Also, the reducing power values of 30 kGy gamma irradiated rosemary were higher than that of non-irradiated (P < 0.05) and the other doses of irradiation could not make any significant difference in reducing power of non-irradiated rosemary. The gamma irradiation of rosemary (at 10–50 kGy), significantly increased the Fe2+ chelating activity compared to non-irradiated rosemary while increasing the absorbed doses reduced Fe2+ chelating activity in rosemary (P < 0.05). There is no significant difference in the rosemary irradiated with 10 kGy and 30 kGy. The gamma irradiated rosemary showed higher antioxidant activities at doses of 30 kGy (79.5 %) and 40 kGy (80 %) with DPPH method, while slightly lower activities were recorded at doses of 30 kGy (34.8 %) and 40 kGy (34.2 %) with ABTS test. Also, results indicate that the 30 kGy might be an effective dose with maximum enhancement in the antioxidant properties of the gamma irradiated rosemary.

中文翻译：

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γ-辐照迷迭香提取物的抗氧化活性值（ABTS 和 DPPH）以及三价铁还原和螯合能力

摘要 本研究旨在评估各种伽马剂量辐照对迷迭香 PBS（磷酸盐缓冲盐水）提取物的抗氧化活性的影响。用10、20、30、40和50 kGy剂量的γ射线辐照迷迭香PBS提取物，并通过DPPH（1,1-二苯基-2-苦基肼）、自由基阳离子ABTS•+研究其抗氧化活性（2,2-Azino-Bis（3-Ethylbenzthiazoline-6-Sulfonic Acid），二铵盐）、铁还原抗氧化能力测定 (FRAP) 和使用亚铁嗪的铁螯合能力。使用 DPPH 和 ABST 两种方法观察到 γ 辐照迷迭香的清除活性在 30 和 40 kGy 时显着更高。此外，30 kGy γ 辐照迷迭香的还原力值高于未辐照的迷迭香（P < 0. 05) 和其他辐照剂量对未辐照迷迭香的还原能力没有任何显着差异。与未辐照的迷迭香相比，迷迭香的伽马辐照（10-50 kGy）显着增加了 Fe2+ 螯合活性，而增加吸收剂量会降低迷迭香中的 Fe2+ 螯合活性（P < 0.05）。10 kGy和30 kGy辐照的迷迭香无显着差异。经伽马辐照的迷迭香在 30 kGy (79.5 %) 和 40 kGy (80 %) 剂量下使用 DPPH 方法显示出更高的抗氧化活性，而在 30 kGy (34.8 %) 和 40 kGy (34.2 %) 剂量下记录的活性略低与 ABTS 测试。此外，结果表明 30 kGy 可能是一个有效剂量，最大程度地增强了伽马辐照迷迭香的抗氧化性能。