Abstract The objective of this study is to determine essential oil, diterpenoid and phenolic contents of Sideritis hololeuca along with their biological activities. Phytol was found to be the main component of the essential oil. Seven known kaurane diterpenoids, siderol (140 mg, 0.16%, w/w), 7-acetoxy sideroxol (15 mg, 0.02%, w/w), eubol (6 mg, 0.01%, w/w), eubotriol (5 mg, 0.03%, w/w), 7-epicandicandiol (3 mg, 0.02%, w/w), ent-7α-acetoxy-18-hydroxykaur-16-ene (5 mg, 0.01%, w/w) and linearol [by liquid chromatography-tandem mass spectrometry (LC-MS/MS)] were determined from the species. Moreover, vanillin (21 mg, 0.10%, w/w), which was not isolated from any Sideritis species earlier, was isolated from an acetone extract of S. hololeuca. Quantitative amounts of some phenolic compounds in n-hexane, dichloromethane, acetone, methanol extracts and infusion and decoction of the plants were also investigated by LC-MS/MS. Antioxidant capacity and acetylcholinesterase, butyrylcholinesterase inhibition effects of the species were evaluated. The extracts of methanol and infusion and decoction of species showed moderate butyrylcholinesterase inhibition activity. The highest inhibition was observed from the decoction of species. The Cu2+ reducing power of infusion was determined as 1.435 mmol TR g−1.

中文翻译：

---

Sideritis hololeuca Boiss & Heldr 的二萜和酚含量。Apud Bentham 具有抗氧化和抗胆碱酯酶活性

摘要 本研究的目的是确定Sideritis hololeuca 的精油、二萜和酚类成分及其生物活性。植物醇被发现是精油的主要成分。七种已知的贝壳杉二萜、siderol（140 mg，0.16%，w/w）、7-乙酰氧基sideroxol（15 mg，0.02%，w/w）、eubol（6 mg，0.01%，w/w）、eubotriol（5 mg, 0.03%, w/w), 7-epicandicandiol (3 mg, 0.02%, w/w), ent-7α-acetoxy-18-hydroxykaur-16-ene (5 mg, 0.01%, w/w)和线性醇 [通过液相色谱-串联质谱 (LC-MS/MS)] 从物种中确定。此外，香草醛（21 毫克，0.10%，w/w），早先没有从任何 Sideritis 物种中分离出来，是从 S. hololeuca 的丙酮提取物中分离出来的。正己烷、二氯甲烷、丙酮、还通过 LC-MS/MS 研究了甲醇提取物以及植物的浸渍和煎煮。评估了该物种的抗氧化能力和乙酰胆碱酯酶、丁酰胆碱酯酶抑制作用。甲醇提取物和浸剂和水煎剂显示出中等的丁酰胆碱酯酶抑制活性。从物种的煎剂中观察到最高的抑制作用。灌注的Cu2+ 还原能力确定为1.435 mmol TR g-1。