Abstract In the review we describe a method for concentration of anionic liposomes with encapsulated water-soluble substances within a small volume via electrostatic liposome adsorption on the surface of polymer particles with grafted cationic chains (spherical polycationic brushes), or cationic microgel particles. Dozens of intact liposomes can be bound to each polymer particle, the resulting polymer/liposome complex does not dissociate into the original components in a physiological solution. This allows fabrication of multi-liposomal complexes (MLCs) with a required ratio of encapsulated substances. Two approaches are discussed for the synthesis of stimuli-sensitive MLCs. The first is to incorporate the conformation switch, morpholinocyclohexanol-based lipid, into the liposomal membrane thus forming pH-sensitive liposomes capable of releasing their cargo when acidifying the surrounding solution. These liposomes complexed with the brushes release encapsulated substances much faster than the uncomplexed liposomes. The second is to adsorb liposomes on cationic thermo-responsive microgels. The resulting MLCs contracts upon heating over a volume phase transition temperature from the swollen to the collapsed state of microgel, thus causing the adsorbed liposomes to change drastically their morphology and release an encapsulated substance. Complexation of anionic liposomes with chitosan microgels and polylactide micelles gives MLCs which degrade in the presence of enzymes down to small particles, 10–15 nm in diameter. A novel promising approach suggests that immobilized liposomes can act as a capacious depot for biologically active compounds and ensure their controllable leakage to surrounding solution.

中文翻译：

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用于受控给药的多功能载体

摘要 在综述中，我们描述了一种通过静电脂质体吸附在具有接枝阳离子链的聚合物颗粒（球形聚阳离子刷）或阳离子微凝胶颗粒表面上，在小体积内浓缩具有包封水溶性物质的阴离子脂质体的方法。数十个完整的脂质体可以与每个聚合物颗粒结合，所得聚合物/脂质体复合物在生理溶液中不会解离成原始成分。这允许制造具有所需包封物质比例的多脂质体复合物 (MLC)。讨论了两种合成刺激敏感 MLC 的方法。第一个是结合构象开关，吗啉代环己醇基脂质，进入脂质体膜，从而形成 pH 敏感脂质体，能够在酸化周围溶液时释放其货物。这些与刷子复合的脂质体比未复合的脂质体更快地释放包封物质。二是将脂质体吸附在阳离子热敏微凝胶上。所得的 MLC 在加热超过体积相变温度时收缩，从微凝胶的膨胀状态到塌陷状态，从而导致吸附的脂质体的形态发生巨大变化并释放出包封的物质。阴离子脂质体与壳聚糖微凝胶和聚丙交酯胶束的络合产生 MLC，在酶的存在下可降解为直径 10-15 nm 的小颗粒。