Abstract Liver injury and disease caused by alcohol is a common complication to human health worldwide. Chamazulene is a natural proazulene with antioxidant and anti-inflammatory properties. This study aims to investigate the hepatoprotective effects of chamazulene against ethanol-induced liver injury in rat models. Adult Wistar rats were orally treated with 50% v/v ethanol (8–12 mL/kg body weight [b.w.]) for 6 weeks to induce alcoholic liver injury. Chamazulene was administered orally to rats 1 h prior to ethanol administration at the doses of 25 and 50 mg/kg b.w. for 6 weeks. Silymarin, a commercial drug for hepatoprotection, was orally administered (50 mg/kg b.w.) for the positive control group. Chamazulene significantly reduced (p < 0.05) the levels of serum alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and malondialdehyde, whereas the levels of antioxidant enzymes (glutathione peroxidase, catalase, and superoxide dismutase) and reduced glutathione were significantly restored (p < 0.05) in contrast to the ethanol model group. The levels of pro-inflammatory cytokines (tumour necrosis factor-α and interleukin-6) were suppressed by chamazulene (p < 0.05) with relevance to ethanol-induced liver injury. Histopathological alterations were convincing in the chamazulene-treated groups, which showed protective effects against alcoholic liver injury. Chamazulene has a significant hepatoprotective effect against ethanol-induced liver injury through alleviation of oxidative stress and prevention of inflammation.

中文翻译：

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Chamazulene 通过减轻氧化应激对大鼠模型中酒精性肝损伤的保肝作用

摘要 酒精引起的肝损伤和疾病是全世界人类健康的常见并发症。Chamazulene 是一种天然的 proazulene，具有抗氧化和抗炎特性。本研究旨在研究查马烯对大鼠模型中乙醇诱导的肝损伤的保肝作用。用 50% v/v 乙醇（8-12 mL/kg 体重 [bw]）口服治疗成年 Wistar 大鼠 6 周以诱导酒精性肝损伤。在乙醇给药前 1 小时，以 25 和 50 毫克/千克体重的剂量向大鼠口服 Chamazulene，持续 6 周。对于阳性对照组，水飞蓟素是一种用于保肝的商业药物，口服给药（50 毫克/千克体重）。Chamazulene 显着降低 (p < 0.05) 血清碱性磷酸酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶、和丙二醛，而与乙醇模型组相比，抗氧化酶（谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶）和还原型谷胱甘肽的水平显着恢复（p < 0.05）。促炎细胞因子（肿瘤坏死因子-α 和白细胞介素-6）的水平被与乙醇诱导的肝损伤相关的chamazulene 抑制（p < 0.05）。chamazulene 治疗组的组织病理学改变令人信服，显示出对酒精性肝损伤的保护作用。Chamazulene 通过减轻氧化应激和预防炎症，对乙醇引起的肝损伤具有显着的保肝作用。和超氧化物歧化酶）和还原型谷胱甘肽与乙醇模型组相比显着恢复（p < 0.05）。促炎细胞因子（肿瘤坏死因子-α 和白细胞介素-6）的水平被与乙醇诱导的肝损伤相关的chamazulene 抑制（p < 0.05）。chamazulene 治疗组的组织病理学改变令人信服，显示出对酒精性肝损伤的保护作用。Chamazulene 通过减轻氧化应激和预防炎症，对乙醇引起的肝损伤具有显着的保肝作用。和超氧化物歧化酶）和还原型谷胱甘肽与乙醇模型组相比显着恢复（p < 0.05）。促炎细胞因子（肿瘤坏死因子-α 和白细胞介素-6）的水平被与乙醇诱导的肝损伤相关的chamazulene (p < 0.05) 抑制。chamazulene 治疗组的组织病理学改变令人信服，显示出对酒精性肝损伤的保护作用。Chamazulene 通过减轻氧化应激和预防炎症，对乙醇引起的肝损伤具有显着的保肝作用。05) 与乙醇引起的肝损伤有关。chamazulene 治疗组的组织病理学改变令人信服，显示出对酒精性肝损伤的保护作用。Chamazulene 通过减轻氧化应激和预防炎症，对乙醇引起的肝损伤具有显着的保肝作用。05) 与乙醇引起的肝损伤有关。chamazulene 治疗组的组织病理学改变令人信服，显示出对酒精性肝损伤的保护作用。Chamazulene 通过减轻氧化应激和预防炎症，对乙醇引起的肝损伤具有显着的保肝作用。