Abstract ASP was used to synthesize FeNPA. They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. The aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNPA and ASP. The antioxidant properties were evaluated using DPPH, ABTS+ and H2O2 assays. FeNPA had higher antioxidant activity comparing to ASP according to DPPH (IC50: 3.48 μg/mL) and ABTS+ (60.52%) assays. Anti-cancer activities of FeNPA and ASP were investigated in breast cancer, melanoma and control cell lines. FeNPA was more cytotoxic than ASP in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNPA had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. ASP also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in ASP, and FeNPA treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in ASP and FeNPA administration. It had been shown that FeNPA regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNPA are also evaluated in animal models in vivo.

中文翻译：

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使用 Asphodelus aestivus Brot 合成的铁纳米颗粒的结构表征、抗氧化和细胞毒性作用。水提物

摘要 ASP 用于合成 FeNPA。它们通过紫外-可见光谱、FT-IR、TEM、SEM、XRD 和 ZP 进行表征。本研究的目的是评估 FeNPA 和 ASP 的体外细胞毒活性和抗氧化活性。使用 DPPH、ABTS+ 和 H2O2 测定法评估抗氧化性能。根据 DPPH (IC50: 3.48 μg/mL) 和 ABTS+ (60.52%) 测定，FeNPA 与 ASP 相比具有更高的抗氧化活性。在乳腺癌、黑色素瘤和对照细胞系中研究了 FeNPA 和 ASP 的抗癌活性。FeNPA 在 MCF-7、MeWo、CHL-1 和 HEL 299 细胞中比 ASP 更具细胞毒性。FeNPA 已经表明，线粒体通过 MDA-MB-231 和细胞 MeWo 中的压力诱导细胞凋亡。ASP 还在 MCF-7 细胞中诱导细胞凋亡 2.23 倍。在 ASP 和 FeNPA 治疗中，激素依赖性 MCF-7 细胞系中的孕酮受体基因表达增加了 10 倍。BCL6、CXCL12、DNAJC15、RB1 和 TPM1 在黑色素瘤癌细胞系中的表达在 ASP 和 FeNPA 给药中显着增加。已经表明 FeNPA 调节基因表达，这在乳腺癌和黑色素瘤细胞系的预后方面可能被认为是重要的，并且表明 FeNPA 调节的基因表达也在体内动物模型中进行了评估。