Aphid species are the most common insect pest infesting wheat plants. The LC values of imidacloprid against aphid spp., ISSR‐PCR molecular markers and biochemical parameters in wheat plant in the present study were carried out. The LC₅₀ of imidacloprid on Rhopalosiphum maidis, Rhopalosiphum padi, Sitobion avenae, and Schzaphis graminum were 71.626, 85.853, 91.05, and 115.806, respectively. While, the biochemical parameters in treated wheat plants including; total protein, RNA, and DNA contents increased in all experimental groups. Moreover, total carbohydrates and soluble sugar also increased in treated wheat seedlings. Also, the genetic diversity studies revealed that among six ISSR primers produced 36 amplified DNA fragments in a molecular weight range of (480–1545 bp). About 28 fragments were monomorphic, and 8 fragments were polymorphic. It can be concluded that the effectiveness of imidacloprid against sucking insects in treated wheat plants may due to few genetic variations and some metabolic changes in the Triticum. aestivum.

中文翻译：

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吡虫啉对某些蚜虫的生物理性作用的测定。以及使用生化参数和ISSR-PCR的小麦（Triticum aestivum L.）。

蚜虫是小麦植物中最常见的害虫。本研究对小麦植株中吡虫啉抗蚜虫的LC值，ISSR-PCR分子标记和生化参数进行了研究。吡虫啉，大叶Rhopalosiphum padi，Sitobion avenae和Schzaphis graminum上吡虫啉的LC ₅₀分别为71.626、85.853、91.05和115.806。同时，处理过的小麦植物的生化参数包括：在所有实验组中，总蛋白质，RNA和DNA含量均增加。此外，处理过的小麦幼苗中的总碳水化合物和可溶性糖也增加。同样，遗传多样性研究表明，在6个ISSR引物中，产生了36个扩增的DNA片段，分子量范围为（480-1545 bp）。大约28个片段是单态的，而8个片段是多态的。可以得出结论，吡虫啉在处理过的小麦植株中对吮吸昆虫的有效性可能是由于小麦中的遗传变异少和代谢改变。小麦。