Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by isobaric tags for relative and absolute quantitation and quantitative polymerase chain reaction (qPCR) assays. A total of 3999 proteins were identified, of which 320 and 181 proteins were differentially expressed at 24 and 96 h postinfection, respectively. The expression levels of 16 selected immune-related differentially expressed proteins (DEPs) were confirmed by qPCR analysis. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that DEPs were significantly associated with complement, inflammation, and antiviral response. The protein–protein interaction network of cytoskeleton-associated proteins, ATPase-related proteins, and parvalbumins from DEPs was shown to be involved in skin immune response. This is first report on the skin proteome profiling of zebrafish against SVCV infection, which will contribute to understand the molecular mechanism of local mucosal immunity in fish.

中文翻译：

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斑马鱼的蛋白质组学分析受到鲤鱼病毒春季病毒血症的挑战，为皮肤抗病毒反应提供了见识。

鲤鱼病毒的春季病毒血症（SVCV）引起了鲤科鱼类的皮肤出血性疾病，但其皮肤免疫应答的分子机制在蛋白质水平上仍不清楚。在本研究中，斑马鱼的蛋白质组学（Danio rerio）用同量异位标签检查对SVCV感染有反应的皮肤，以进行相对和绝对定量以及定量聚合酶链反应（qPCR）分析。共鉴定出3999个蛋白质，其中分别在感染后24和96 h差异表达320和181个蛋白质。通过qPCR分析确认了16种选择的免疫相关差异表达蛋白（DEP）的表达水平。此外，《基因本体论》和《京都议定书》的基因与基因组富集分析表明，DEP与补体，炎症和抗病毒反应显着相关。细胞骨架相关蛋白，ATPase相关蛋白和DEPs小白蛋白的蛋白-蛋白相互作用网络被证明与皮肤免疫反应有关。